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首页> 外文期刊>Journal of Phytopathology >Sporulation, Inoculation Methods and Pathogenicity of Ustilaginoidea albicans, the Cause of White Rice False Smut in China
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Sporulation, Inoculation Methods and Pathogenicity of Ustilaginoidea albicans, the Cause of White Rice False Smut in China

机译：白米假丝黑穗病的形成原因：白色念珠菌的形成，接种方法及致病性

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摘要

Sporulation and inoculation methods were studied to determine the pathogenicity of Ustilaginoidea albicans S Wang and JK Bai, the cause of white rice false smut, isolated in China. High sporulation was obtained using solid culture, liquid fermentation and double layer incubation system. In liquid culture, the culture media composition contributed to observed differences in sporulation. Potato sucrose broth was the optimal media to promote conidia production, increasing the concentration to up to 4.810 super(8)spores-ml. Inoculation by injection and spraying resulted in disease development, demonstrating that conidia of white rice false smut are virulent to rice plants, despite apparent differences in disease incidence. Estimates of mean disease severity were higher for injection than for spraying as assessed by percent infected panicles. The two methods resulted in disease severity ranging from 12.0 to 28.7% and 8.2 to 20.7%, respectively. Low temperature exposure after inoculation had a strong stimulatory effect on disease development.
机译：研究了孢子形成和接种方法，以确定在中国分离的白米假丝菌的病原，Ustilaginoidea albicans S Wang和JK Bai。使用固态培养，液体发酵和双层培养系统可获得较高的孢子形成率。在液体培养中，培养基成分有助于观察到孢子形成的差异。马铃薯蔗糖肉汤是促进分生孢子产生的最佳培养基，可将其浓度提高到高达4.810 super（8）spores-ml。通过注射和喷雾接种导致疾病发展，表明尽管疾病发病率存在明显差异，但白稻假菌的分生孢子对稻株有毒害。根据感染穗的百分率评估，注射用平均疾病严重程度的估计值高于喷洒。两种方法导致的疾病严重程度分别为12.0至28.7％和8.2至20.7％。接种后的低温暴露对疾病的发展有很强的刺激作用。

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