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首页> 外文期刊>Journal of Phytopathology >A Quantitative Polymerase Chain Reaction Assay for the Detection of Polyscytalum pustulans, the Cause of Skin Spot Disease of Potato
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A Quantitative Polymerase Chain Reaction Assay for the Detection of Polyscytalum pustulans, the Cause of Skin Spot Disease of Potato

机译：定量聚合酶链反应分析法检测脓疱，马铃薯马铃薯斑病的病因

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摘要

Skin spot disease of potato caused by the pathogen Polyscytalum pustulans is likely to become more important with the withdrawal of 2-aminobutane as a fungicide, and new methods of control will need to be found. As part of a disease control strategy, it will be necessary to study the disease in more detail, to utilize host resistance and to identify stocks where problems are likely to arise. Existing methods for the detection and quantification of P. pustulans are time-consuming and require specific expertise. Real-time PCR assays have been developed for many pathogens of potato and have subsequently been used as tools for the study of the epidemiology and control of disease. The development of a real-time PCR assay for the detection and quantification of P. pustulans is described. The specificity of the assay was demonstrated and detection was shown to be reliable at levels as low as 20-250 fg/ol DNA, (equivalent to 60-680 pg DNA/g) in soil and on symptomless tubers at attogram (ag) levels. These values are in line with previously developed tests.
机译：随着2-氨基丁烷作为杀真菌剂的撤离，由病原菌脓毒杆菌引起的马铃薯皮斑病可能变得更加重要，并且将需要寻找新的控制方法。作为疾病控制策略的一部分，有必要更详细地研究疾病，利用宿主抗药性并找出可能出现问题的种群。现有的用于检测和定量脓疱疮的方法费时并且需要专门知识。已经开发了用于马铃薯的许多病原体的实时PCR测定法，并且随后将其用作研究流行病学和疾病控制的工具。描述了实时PCR测定法的发展，用于检测和定量脓疱疮杆菌。证明了该测定方法的特异性，并且在低至20-250 fg / ol DNA（相当于60-680 pg DNA / g）的土壤和无症状块茎（attogram（ag））水平下检测是可靠的。这些值与以前开发的测试一致。

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