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首页> 外文期刊>Journal of phycology >Synergistic effects of light, temperature, and nitrogen source ontranscription of genes for carbon and nitrogen metabolism in thecentric diatom Thalassiosira pseudonana (Bacillariophyceae)
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Synergistic effects of light, temperature, and nitrogen source ontranscription of genes for carbon and nitrogen metabolism in thecentric diatom Thalassiosira pseudonana (Bacillariophyceae)

机译：光，温度和氮源对中心硅藻拟杆菌（拟杆菌）碳和氮代谢基因转录的协同效应

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摘要

The effects of nitrogen source, temperature, and light level on transcription of five genes from three pathways of carbon and nitrogen metabolism were determined for the centric diatom Thalassiosira pseudonana (Hustedt) Hasle et Heimdal. The targeted genes were NR (nitrate reductase) and GSII (glutamine synthetase II), both required for nitrate utilization; PGP (phosphoglycolate phosphatase) and GDCT (glycine decarboxylase T-protein), both required for photorespiration; and SBP (sedoheptulose 1,7-bisphosphatase), required for carbon fixation (Calvin cycle). Quantitative reverse transcriptase PCR was used to compare transcript abundances for these genes from T. pseudonana cells grown at two light intensities (50 or 300 mu mol photons.m super(-2).s super(-1)), at two temperatures (12 or 22 degree C), and on two different nitrogen sources (nitrate or ammonium). Transcript abundance (normalized to 18S rRNA) was higher for all five genes at the higher light intensity. At 12 degree C, fewer transcripts were detected for the Calvin cycle gene, whereas more transcripts were detected for the photorespiration and nitrate utilization genes. Nitrogen source affected transcript accumulation of the photorespiration and nitrate utilization genes but not the Calvin cycle gene. Extracellular concentrations of glycolate, a photorespiratory-specific product, were determined under the high light conditions and were found to decrease at 12 degree C and when cells were grown on ammonium. A hypothetical model is presented to explain the patterns of transcript accumulation and glycolate release in terms of energy balance within the cell.
机译：确定了中心硅藻拟硅藻拟南芥（Hustedt）Hasle et Heimdal的氮源，温度和光照水平对来自碳和氮代谢三个途径的五个基因转录的影响。靶向基因是NR（硝酸盐还原酶）和GSII（谷氨酰胺合成酶II），两者都需要硝酸盐利用。光呼吸所需的PGP（磷酸甘氨酸磷酸酶）和GDCT（甘氨酸脱羧酶T蛋白）;和碳固定（卡尔文循环）所需的SBP（七庚糖1,7-双磷酸酶）。定量逆转录酶PCR用于比较来自两个温度（50或300μmol光子.m super（-2）.s super（-1））在两个温度（50或300μmol光子.m super（-2）.s super（-1））上生长的假单胞菌细胞中这些基因的转录丰度。 12或22摄氏度），并使用两种不同的氮源（硝酸盐或铵盐）。在较高的光照强度下，所有五个基因的转录本丰度（标准化为18S rRNA）都较高。在12摄氏度下，卡尔文循环基因的转录物较少，而光呼吸和硝酸盐利用基因的转录物却更多。氮源影响光呼吸和硝酸盐利用基因的转录本积累，但不影响卡尔文循环基因。在强光条件下测定了光呼吸特异性产物乙醇酸的细胞外浓度，发现在12摄氏度和在铵上生长的细胞中，其浓度会降低。提出了一个假想模型来解释转录本积累和乙醇酸释放的模式，涉及细胞内的能量平衡。

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