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首页> 外文期刊>Journal of pesticide science >Phylogenetic diversity and sensitivity to MBI and QoI fungicides of Magnaporthe oryzae in Taiwan
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Phylogenetic diversity and sensitivity to MBI and QoI fungicides of Magnaporthe oryzae in Taiwan

机译：台湾稻瘟病菌的系统发育多样性及其对MBI和QoI杀真菌剂的敏感性

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摘要

Magnaporthe oryzae (Anamorph: Pyricularia oryzae), the causal agent of rice blast disease, is the major pathogen that reduces the yield of rice worldwide. Melanin biosynthesis inhibitors (MBIs) and strobilurins (QoIs) are common fungicides used to control the disease. One hundred and three M. oryzae isolates from different rice-production areas in Taiwan were evaluated for their molecular phylogeny and sensitivity to MBI and QoI fungicides. Molecular phylogenetic analysis showed that all M. oryzae isolates could be classified into nine genetic groups (A-I) based on the DNA polymorphism amplified by CNS1/MP primers. Predominant ones are groups A (72 isolates) and B (15 isolates). Fungicide tests showed that M. oryzae isolates were sensitive to MBIs (carpropamid and tricyclazole) and less sensitive to QoIs (kresoxim-methyl and azoxystrobin). Amplifying the cytochrome b (cyt b) gene revealed that isolates with low sensitivity to QoIs did not correspond with mutation at codon 129 or 143 in the cyt b gene.
机译：稻瘟病的致病因子是稻瘟病菌（Magnaporthe oryzae），是导致全世界稻米减产的主要病原体。黑色素生物合成抑制剂（MBIs）和嗜球果伞素（QoIs）是用于控制该疾病的常见杀菌剂。对台湾不同水稻生产地区的一百三十三个米曲霉分离株的分子系统发育以及对MBI和QoI杀菌剂的敏感性进行了评估。分子系统发育分析表明，基于CNS1 / MP引物扩增的DNA多态性，所有米曲霉分离株均可分为9个遗传组（A-1）。主要的是A组（72个菌株）和B组（15个菌株）。杀菌剂测试显示，米曲霉分离株对MBI（丙酰胺和三环唑）敏感，而对QoI（克罗索辛甲基和嘧菌酯）敏感。扩增细胞色素b（cyt b）基因表明，对QoIs敏感性较低的分离株与cyt b基因中第129或143位密码子的突变不符。

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