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首页> 外文期刊>Journal of pesticide science >Effects of different classes of attractarits, cochliophilin A and N-(E)-feruloyl-4-O-methyldopamine, on the response of Aphanomyces cochlioides zoospores in their chemoattraction and activation of motility linked with intracellular cAMP
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Effects of different classes of attractarits, cochliophilin A and N-(E)-feruloyl-4-O-methyldopamine, on the response of Aphanomyces cochlioides zoospores in their chemoattraction and activation of motility linked with intracellular cAMP

机译：不同种类的吸引剂，亲脂蛋白A和N-（E）-阿魏酰-4-O-甲基多巴胺对Aphanomyces cochlioides游动孢子的化学吸引和与细胞内cAMP相关的运动活化的响应

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摘要

Cochliophilin A (5-hydroxy-6,7-methylenedioxyflavone, 1) and N-(E)-feruloyl-4-O-methyldopamine (2) are naturally occurring host-specific chemoattractants for Aphanomyces cochlioides zoospores. In a cross-competition assay, compound 1 (4 fmol) applied to diatomite particles clearly attracted A. cochlioides zoospores in an aqueous solution of excessive compound 2 (1X 10~(-6) M) that could mask a concentration gradient of 1 dispersed from the particles. Similarly, 2 (40 fmol) on particles also attracted A. cochlioides zoospores in an aqueous solution of 1 (1X10~(-6)M). In addition, compound 2 on the particles did not attract the zoospores in an aqueous solution containing 1X10~(-6)M NADP~+, while 1 clearly attracted zoospores in the same solution. These results allowed us to speculate that compounds 1 and 2 do not share receptors. The chemosensory system for 1 is probably for host recognition of A. cochlioides zoospores, while the system for 2 is linked to cell differentiation.
机译：Cochliophilin A（5-羟基-6,7-亚甲基二氧基黄酮，1）和N-（E）-阿魏酰-4-O-甲基多巴胺（2）是天然的寄主特定趋化剂，用于Aphanomyces cochlioides游动孢子。在交叉竞争试验中，应用于硅藻土颗粒的化合物1（4 fmol）在过量的化合物2（1X 10〜（-6）M）的水溶液中明显吸引了球形曲霉游动孢子，这可能掩盖了分散的1的浓度梯度。从粒子。类似地，在1（1X10〜（-6）M）的水溶液中，颗粒上的2（40 fmol）也吸引了球形曲霉游动孢子。此外，在含有1X10〜（-6）M NADP〜+的水溶液中，颗粒上的化合物2没有吸引游动孢子，而在相同溶液中1明显吸引了游动孢子。这些结果使我们推测化合物1和2不共享受体。用于1的化学感应系统可能是宿主识别A. cochlioides游动孢子，而用于2的系统则与细胞分化有关。

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