...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Biophysical Chemistry: An International Journal Devoted to the Physical Chemistry of Biological Phenomena >Practical biophysics: Sensors for rapid detection of biological targets utilizing target-induced oligonucleotide annealing.
【24h】

Practical biophysics: Sensors for rapid detection of biological targets utilizing target-induced oligonucleotide annealing.

机译：实用的生物物理学：利用目标物诱导的寡核苷酸退火快速检测生物目标物的传感器。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Detection and quantitation of biomolecules is one of the most commonly performed measurements in biomedical research and clinical diagnostics. There is high demand for convenient, rapid and sensitive biomolecule detection methodologies. In this review we discuss a family of sensors that have been developed in our laboratory that share a common simple biophysical mechanism of action and that are capable of rapid detection of a diverse range of biological targets. The sensors generate fluorescence signal in the presence of the target molecule through target-induced association of short fluorochrome-labeled complementary oligonucleotides that are attached to target recognition elements of the sensors (antibodies, aptamers, etc.) via nanometer scale flexible linkers. This sensor design can be used for detecting proteins, antibodies, nucleic acids and whole cells. The assays using these sensors require only adding a sample to the sensor mix followed by simple fluorescence intensity readout. The simplicity, the speed of detection and the potential for miniaturization are the main assets of these sensors.
机译：生物分子的检测和定量是生物医学研究和临床诊断中最常执行的测量之一。对于方便，快速和灵敏的生物分子检测方法有很高的要求。在这篇综述中，我们讨论了在我们的实验室中开发的一系列传感器，它们具有共同的简单生物物理作用机制，并且能够快速检测多种生物靶标。传感器通过靶标诱导的短荧光染料标记的互补寡核苷酸的靶标诱导缔合，在靶分子存在下产生荧光信号，所述寡核苷酸通过纳米级柔性接头连接到传感器的靶标识别元件（抗体，适体等）。该传感器设计可用于检测蛋白质，抗体，核酸和整个细胞。使用这些传感器的测定只需将样品添加到传感器混合物中，然后简单地读取荧光强度即可。这些传感器的主要优点在于其简单性，检测速度和小型化潜力。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号