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首页> 外文期刊>Journal of Physics, D. Applied Physics: A Europhysics Journal >Quantitative estimation of biological cell concentration suspended in aqueous medium by using dielectrophoretic impedance measurement method
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Quantitative estimation of biological cell concentration suspended in aqueous medium by using dielectrophoretic impedance measurement method

机译：用介电泳阻抗测量法定量估算悬浮在水性介质中的生物细胞浓度

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摘要

We describe a new technique that realizes the quantitative estimation of the biological cell concentration in an aqueous medium. The proposed dielectrophoretic impedance measurement method utilizes the positive dielectrophoretic force to capture suspended biological particles onto an interdigitated micro-electrode array in pearl-chain formation. Higher cell concentration results in faster development of the pearl chains, which are electrically connected in parallel within the electrode gap to increase the conductance and capacitance between the electrodes. By monitoring temporal variation of the electrical impedance, it is possible to quantitatively evaluate the cell population according to a theoretical model of the cell collection process. It has been demonstrated that a suspension liquid of Escherichia coli could be accurately assayed in about 10 min at 10~5 cm~(-3) concentration.
机译：我们描述了一种新技术，可以实现对水性介质中生物细胞浓度的定量估计。拟议的介电电泳阻抗测量方法利用正介电电泳力将悬浮的生物颗粒捕获到形成珍珠链的交错式微电极阵列上。较高的电池浓度会导致珍珠链更快地发展，这些珍珠链在电极间隙内并联电连接以增加电极之间的电导和电容。通过监视电阻抗的时间变化，可以根据细胞收集过程的理论模型定量评估细胞数量。已经证明，可以在约10分钟内以10〜5 cm〜（-3）的浓度准确测定大肠杆菌的悬浮液。

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