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首页> 外文期刊>Journal of Plant Biology >Effect of La(NO3)(3) and Ce(NO3)(3) on shoot induction and seedling growth of in vitro cultured anoectochilus roxburghii
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Effect of La(NO3)(3) and Ce(NO3)(3) on shoot induction and seedling growth of in vitro cultured anoectochilus roxburghii

机译：La（NO3）（3）和Ce（NO3）（3）对离体培养的拟金线莲芽诱导和幼苗生长的影响

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摘要

Anoectochilus roxburghii, a highly valuable medicinal plant species, is threatened in its native habitat. To ensure the sustainability of this useful resource, we studied the effect of La(NO3)(3) and Ce(NO3)(3) on plant tissue culturepropagated A. roxburghii. Apical buds, mid-stem segments, and basal rhizome segments were taken from aseptic seedlings of A. roxburghii and cultured in vitro on half-strength Murashige-Skoog medium containing different concentrations (up to 5.0 mg/L) of La(NO3)(3) or Ce(NO3)(3). After 100 d of culturing, average heights of plantlets derived from apical buds were 6.0 cm with 5.0 mg/L La(NO3)(3) and 6.2 cm with 2.0 mg/L Ce(NO3)(3), which respectively increased by 28.0% and 32.0% as compared with that in the non-treated control group. The optimum concentration for shoot induction from mid-stem segments was 1.0 mg/L Ce(NO3)(3) which had a better proliferation times of 1.5-fold and an average length of 3.0 cm compared with 1.0-fold and 2.2 cm in the control group. Optimum growth from basal rhizome segments was achieved on media supplemented with 3.0 mg/L Ce(NO3)(3), which provided a better proliferation times of 5.1-fold and an average shoot length of 4.5 cm compared with corresponding control values of 2.0-fold and 3.5 cm. Our results showed that La(NO3)(3) and Ce(NO3)(3) can accelerate A. roxburghii regeneration, which was probably due to the effect on chlorophyll contents, enzymes activity (superoxide dismutase, catalase and peroxidase) and malonldialedhyde contents caused by the addition of La(NO3)(3) or Ce(NO3)(3).
机译：珍贵的药用植物金线莲（Anoectochilus roxburghii）在其本地栖息地受到威胁。为了确保此有用资源的可持续性，我们研究了La（NO3）（3）和Ce（NO3）（3）对植物组织培养繁殖的拟南芥的影响。根尖芽，中茎节段和基础根茎节段取自A.roxburghii的无菌幼苗，并在半浓度的Murashige-Skoog培养基中体外培养，该培养基含有不同浓度（最高5.0 mg / L）的La（NO3）（ 3）或Ce（NO3）（3）。培养100 d后，根尖芽的平均苗高分别为5.0 cm / L La（NO3）（3）为6.0 cm和2.0 mg / L Ce（NO3）（3）为6.2 cm，分别增加了28.0与未治疗的对照组相比，％和32.0％。茎干中段诱导芽的最佳浓度为1.0 mg / L Ce（NO3）（3），其增殖时间为1.5倍，平均长度为3.0厘米，而相比之下，其为1.0倍和2.2厘米。控制组。在补充了3.0 mg / L Ce（NO3）（3）的培养基上，可以从基础根状茎节段中获得最佳生长，与相应的对照值2.0-2.0相比，该培养基具有更好的5.1倍增殖时间和4.5 cm的平均芽长折叠和3.5厘米。我们的结果表明，La（NO3）（3）和Ce（NO3）（3）可以促进罗氏沼虾的再生，这可能是由于对叶绿素含量，酶活性（超氧化物歧化酶，过氧化氢酶和过氧化物酶）和丙二醛含量的影响由添加La（NO3）（3）或Ce（NO3）（3）引起的。

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