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首页> 外文期刊>Journal of Plant Biology >Callus Culture and High Frequency Plantlet Regeneration Technique - an Efficient Tool to Replenish Centella asiatica (L.) Population in Wild
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Callus Culture and High Frequency Plantlet Regeneration Technique - an Efficient Tool to Replenish Centella asiatica (L.) Population in Wild

机译：愈伤组织培养和高频植株再生技术-补充野生积雪草的有效工具

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摘要

Efforts were made to achieve indirect regeneration in Centella asiatica (L.), a medicinal plant of high economic importance. Successful callus induction and subsequent organogenesis was obtained using leaf and petiole explants. Irrespective of explants used, Murashige and Skoog (MS) media supplemented with benzylaminopurine (BAP) and naphthaleneacetic acid (NAA) were more conducive for callusing than that with BAP alone. Leaf explants were found to be superior towards callus induction response. On anaverage 86.67% of leaf explants exhibited callusing on MS media supplemented with BAP (2 mg/1) and NAA (0.1 mg/1). Shoot organogenesis occurred in the same kind of media. Maximum of 30.67 shoots of 5.66 cm length were obtained from calli derived from petiole on the same kind of media. Cent per cent shoots could induce roots in quarter strength MS media containing 0.3 mg/1 indole-3-butyric acid (IBA). All the rooted plantlets were hardened on substrate containing a mixture of vermicompost and sand (4 :1).
机译：努力在具有重要经济意义的药用植物积雪草（L.）中实现间接再生。使用叶和叶柄外植体获得成功的愈伤组织诱导和随后的器官发生。不管使用何种外植体，补充了苄氨基嘌呤（BAP）和萘乙酸（NAA）的Murashige和Skoog（MS）培养基都比单独使用BAP更有利于call愈。发现叶外植体优于愈伤组织诱导反应。平均而言，在补充了BAP（2 mg / 1）和NAA（0.1 mg / 1）的MS培养基上，有86.67％的叶子外植体表现出愈伤组织。芽器官发生在相同的培养基中发生。在相同种类的培养基上，从叶柄衍生的愈伤组织中最多获得30.67个芽，长度为5.66 cm。百分之几的芽可以诱导四分之一强度的MS​​培养基中的根，该培养基含有0.3 mg / 1吲哚-3-丁酸（IBA）。将所有生根的小苗在包含ver粉和沙子（4：1）的混合物的基质上硬化。

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