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首页> 外文期刊>Journal of Plant Pathology >ELIMINATION OF GRAPEVINE LEAFROLL-ASSOCIATED VIRUS 1 AND GRAPEVINE RUPESTRIS STEM PITTING-ASSOCIATED VIRUS FROM GRAPEVINE cv. AGIORGITIKO, AND A MICROPROPAGATION PROTOCOL FOR MASS PRODUCTION OF VIRUS-FREE PLANTLETS
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ELIMINATION OF GRAPEVINE LEAFROLL-ASSOCIATED VIRUS 1 AND GRAPEVINE RUPESTRIS STEM PITTING-ASSOCIATED VIRUS FROM GRAPEVINE cv. AGIORGITIKO, AND A MICROPROPAGATION PROTOCOL FOR MASS PRODUCTION OF VIRUS-FREE PLANTLETS

机译：从葡萄柚cv中消除葡萄柚叶状病毒相关病毒1和葡萄柚RUPESTRIS STEM点蚀相关病毒。 AGIORGITIKO和用于无病毒植物大规模生产的微繁殖协议

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摘要

Grapevine leafroll-associated virus 1 (GLRaV-1) and Grapevine rupestris stem pitting-associated virus (GRSPaV) were eradicated from Vitis vinifera L cv. Agiorgitiko by combining in vitro thermotherapy and tissue culture. GRSPaV is known to be quite recalcitrant to elimination, whereas GLRaV-1 is more easily knocked out. In this study, the effectiveness of two different virus elimination techniques, including meristem- and shoot-tip culture, was evaluated. Results showed that meristem-tip culture combined with thermotherapy was the most effective for eliminating both viruses as confirmed by nested RT-PCR assays. Success rate for GLRaV-1 (91.2%) was higher than for GRSPaV-1 (67.6%). The ratio of virus elimination to survival was higher for meristem-tip culture than for shoot tips (1.108 and 0.469 respectively). The effect of six basal media on in vitro shoot proliferation of virus-free explants of cv. Agiorgitiko was also studied and woody plant medium (WPM) proved to be the most effective. The presence of cytokinin 6-benzyladenine (BA) alone resulted in chlorotic plantlets, while Supplementation with the auxin naphthaleneacetic acid (NAA) enhanced proliferation rate. Root induction at two temperature regimes (22 +/- 2 degrees C and 26 +/- 21 degrees C) revealed that higher temperature was more effective in the presence of IBA (indole-3-butyric acid) rather than NAA.
机译：从葡萄（Vitis vinifera L cv）上消灭了与葡萄卷叶相关的病毒1（GLRaV-1）和与葡萄藤茎麻点相关的病毒（GRSPaV）。 Agiorgitiko将体外热疗和组织培养相结​​合。已知GRSPaV非常难以消除，而GLRaV-1更容易被淘汰。在这项研究中，评估了包括分生组织和梢尖培养在内的两种不同病毒消除技术的有效性。结果表明，分巢RT-PCR分析证实，分生组织顶端培养与热疗相结合对消除两种病毒最有效。 GLRaV-1的成功率（91.2％）高于GRSPaV-1的成功率（67.6％）。分生组织顶端培养的病毒清除率与存活率之比高于枝梢显示（分别为1.108和0.469）。六种基础培养基对无病毒cv外植体离体芽增殖的影响。还对Agiorgitiko进行了研究，事实证明木本植物培养基（WPM）是最有效的。单独存在细胞分裂素6-苄基腺嘌呤（BA）会导致褪绿植物苗，而补充生长素萘乙酸（NAA）则可提高增殖速率。在两种温度下（22 +/- 2摄氏度和26 +/- 21摄氏度）的根诱导表明，在存在IBA（吲哚-3-丁酸）而不是NAA的情况下，更高的温度更有效。

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