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首页> 外文期刊>Journal of Plant Physiology >Phenotypic diversity of diploid and haploid Emiliania huxleyi cells and of cells in different growth phases revealed by comparative metabolomics
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Phenotypic diversity of diploid and haploid Emiliania huxleyi cells and of cells in different growth phases revealed by comparative metabolomics

机译：比较代谢组学揭示了二倍体和单倍体Emilia huxleyi细胞以及处于不同生长阶段的细胞的表型多样性

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摘要

In phytoplankton a high species diversity of microalgae co-exists at a given time. But diversity is not only reflected by the species composition. Within these species different life phases as well as different metabolic states can cause additional diversity. One important example is the coccolithophore Emiliania huxleyi. Diploid cells play an important role in marine ecosystems since they can form massively abundant algal blooms but in addition the less abundant haploid life phase of E. huxleyi occurs in lower quantities. Both life phases may fulfill different functions in the plankton. We hypothesize that in addition to the functional diversity caused by this life phase transition the growth stage of cells can also influence the metabolic composition and thus the ecological impact of E. huxleyi. Here we introduce a metabolomic survey in dependence of life phases as well as different growth phases to reveal such changes. The comparative metabolomic approach is based on the extraction of intracellular metabolites from intact microalgae, derivatization and analysis by gas chromatography coupled to mass spectrometry (GC-MS). Automated data processing and statistical analysis using canonical analysis of principal coordinates (CAP) revealed unique metabolic profiles for each life phase. Concerning the correlations of metabolites to growth phases, complex patterns were observed. As for example the saccharide mannitol showed its highest concentration in the exponential phase, whereas fatty acids were correlated to stationary and sterols to declining phase. These results are indicative for specific ecological roles of these stages of E. huxleyi and are discussed in the context of previous physiological and ecological studies. (C) 2014 Elsevier GmbH. All rights reserved.
机译：在浮游植物中，在给定的时间共存着高种类的微藻。但是多样性不仅由物种组成反映出来。在这些物种中，不同的生命阶段以及不同的代谢状态会导致其他多样性。一个重要的例子是coccolithophore Emiliania huxleyi。二倍体细胞在海洋生态系统中起着重要作用，因为它们可以形成大量的藻华，但除此之外，虎x的单倍体生命期较少，数量较少。这两个生命阶段可能在浮游生物中履行不同的职能。我们假设，除了由这一生命阶段转变引起的功能多样性以外，细胞的生长阶段还可以影响新孢子虫的代谢组成，进而影响其生态影响。在这里，我们根据生活阶段以及不同的成长阶段来介绍代谢组学调查，以揭示这种变化。比较代谢组学方法基于从完整微藻中提取细胞内代谢物，衍生化和通过气相色谱-质谱联用（GC-MS）分析。使用主坐标（CAP）的规范分析进行的自动数据处理和统计分析揭示了每个生命阶段的独特代谢谱。关于代谢物与生长期的相关性，观察到复杂的模式。例如，糖甘露醇在指数期显示出最高浓度，而脂肪酸与固定相相关，而固醇与下降相相关。这些结果表明E. huxleyi这些阶段的特定生态作用，并在先前的生理和生态研究的背景下进行了讨论。 （C）2014 Elsevier GmbH。版权所有。

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