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A simple, fast and inexpensive method to assess salt stress tolerance of aerial plant part: Investigations in the mandarin group

机译:一种评估空中植物部分耐盐胁迫能力的简单,快速且廉价的方法:国语组中的研究

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For grafted plants, salt stress tolerance of the aerial plant part is poorly documented. Thus, we developed a simple, fast and inexpensive method to identify tolerant genotypes. Twigs of 14 mandarin accessions that we previously analyzed as seedlings were cut in solution to prevent embolism and were then evaluated in salt stress condition for a week. Physiological parameters such as gas exchanges, leaf Cl- and Na+, as well as the presence of H2O2 and the activity of enzymes involved in ROS synthesis and detoxification processes were analyzed. One accession known to be tolerant as rootstock was shown to be sensitive with limited Cl- translocation from the solution to the shoot while sensitive accessions when grown as seedlings presented limited wilting symptoms and accumulated large leaf Cl- content. A model is proposed to explain the different strategies of the plant to cope with high toxic ion content. This method allows separation of the root compartment, where ion exclusion mechanisms may exist and have an impact on the salt stress tolerance of the whole plant. (C) 2015 Elsevier GmbH. All rights reserved.
机译:对于嫁接植物,气生植物部分的耐盐胁迫性文献很少。因此,我们开发了一种简单,快速且廉价的方法来鉴定耐受基因型。在溶液中切掉我们先前分析的14种普通话种的树枝,以防止栓塞,然后在盐胁迫条件下评估一周。分析了生理参数,例如气体交换,叶片的Cl-和Na +以及H2O2的存在以及ROS合成和解毒过程中涉及的酶的活性。一种已知可以耐受砧木的种质对Cl-从溶液到茎的易位敏感,而当幼苗生长时出现敏感的种质则出现了有限的萎symptoms症状并积累了较大的Cl-含量。提出了一个模型来解释植物应对高毒性离子含量的不同策略。这种方法可以分离根室,在该处可能存在离子排斥机制,并影响整个植物的耐盐胁迫性。 (C)2015 Elsevier GmbH。版权所有。

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