Isolation and characterization of an aspartic proteinase gene from cowpea (Vigna unguiculata L. Walp.)

de Carvalho MHC; Pham-Thi AT; Gareil M; dArcy-Lameta A; Fodil YZ

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Isolation and characterization of an aspartic proteinase gene from cowpea (Vigna unguiculata L. Walp.)

机译：cow豆天冬氨酸蛋白酶基因的分离和鉴定（Vigna unguiculata L.Walp。）

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A cowpea (Vigna unguiculata cv. EPACE-1) aspartic proteinase (AP) gene was isolated by genomic library screening. Sequence analysis shows that this AP gene follows the same pattern of intron/exon number and organization as the other isolated plant AP genes, which are distinct from other solved AP genes. Northern blot analysis revealed that cowpea AP accumulates in leaves and stems but not in roots, indicating tissue-specific expression. An increased accumulation of transcripts during senescence suggests enzyme involvement in this process.

机译：通过基因组文库筛选分离了pea豆（Vigna unguiculata cv。EPACE-1）天冬氨酸蛋白酶（AP）基因。序列分析表明，该AP基因与其他分离的植物AP基因遵循相同的内含子/外显子数量和组织模式，这与其他解决的AP基因不同。 Northern印迹分析显示revealed豆AP在叶和茎中积累，但在根中不积累，表明组织特异性表达。衰老过程中转录物积累的增加表明酶参与了该过程。

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《Journal of Plant Physiology》 |2004年第8期|共6页
作者
de Carvalho MHC; Pham-Thi AT; Gareil M; dArcy-Lameta A; Fodil YZ;
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正文语种 eng
中图分类植物学;
关键词
Aspartic proteinase; Cowpea; Genomic library screening; Expression; In-vitro; Elements; Identification; Organization; Senescence; Expression; Oryzasin; Protease; Encodes; Barley;

机译：天冬氨酸蛋白酶;牛ow;基因组文库筛选;表达;体外;元件;鉴定;组织;衰老;表达;Oryzasin;蛋白酶;编码;大麦;

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1. Isolation and characterization of an aspartic proteinase gene from cowpea (Vigna unguiculata L. Walp.) [J] . de Carvalho MHC, Pham-Thi AT, Gareil M, Journal of Plant Physiology . 2004,第8期

机译：cow豆天冬氨酸蛋白酶基因的分离和鉴定（Vigna unguiculata L.Walp。）
2. Isolation and characterization of plant growth promoting bacteria from non-rhizospheric soil and their effect on cowpea (Vigna unguiculata (L.) Walp.) seedling growth [J] . C. K. Deepa, Syed G. Dastager, Ashok Pandey World Journal of Microbiology and Biotechnology . 2010,第7期

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5. Trap Cropping - Development and Deployment for control of the Brown Marmorated Stink bug (Halyomorpha halys [Stal]) on Cowpea (Vigna unguiculata L. Walp.). [D] . Jeje, Olusola. 2016

机译：陷阱作物-Development豆（Vigna unguiculata L.Walp。）上控制和控制褐褐臭臭虫（Halyomorpha halys [Stal]）的开发和部署。
6. Characterization of seed storage proteins in high protein genotypes of cowpea Vigna unguiculata (L.) Walp. [O] . Prachi Gupta, Rohtas Singh, S. Malhotra, 2010

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Isolation and characterization of an aspartic proteinase gene from cowpea (Vigna unguiculata L. Walp.)

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