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首页> 外文期刊>Biophysical Chemistry: An International Journal Devoted to the Physical Chemistry of Biological Phenomena >Conformation and dynamics of DNA molecules during photoreversible condensation.
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Conformation and dynamics of DNA molecules during photoreversible condensation.

机译：光可逆缩合过程中DNA分子的构象和动力学。

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摘要

Direct observation of the mechanism and dynamics of photo-initiated DNA compaction and re-expansion using a light-responsive cationic surfactant has been achieved with fluorescence microscopy. The surfactant undergoes a reversible photoisomerization upon exposure to visible (trans isomer, relatively hydrophobic) or UV (cis isomer, relatively hydrophilic) light. Thus, surfactant binding to DNA and the DNA condensation that result can both be initiated and controlled with light illumination. The inherent kinetics of DNA conformational changes, directly visualized following the in situ light "trigger" of surfactant photoisomerization, are found to occur at rates of approximately 9 microm/s or 240 kbp/s, at or near rates that can be achieved in natural processes. Furthermore, observation of photo-initiated DNA compaction, free of the effects of shear or mixing, provides evidence of a condensation mechanism that nucleates at the ends of the macromolecule. Ethidium bromide displacement studies, employed to gain insight on the mode of interaction between the photo-surfactant and DNA, also reveal the importance of both electrostatic and hydrophobic forces in surfactant binding and DNA condensation.
机译：通过荧光显微镜已经可以直接观察到使用光响应性阳离子表面活性剂进行光引发的DNA压缩和再扩展的机理和动力学。当暴露于可见光（反式异构体，相对疏水）或UV光（顺式异构体，相对亲水）时，表面活性剂经历可逆的光异构化。因此，表面活性剂与DNA的结合以及由此产生的DNA缩合可以通过光照来引发和控制。发现在表面活性剂光异构化的原位光“触发”之后直接可视化的DNA构象变化的内在动力学以大约9 microm / s或240 kbp / s的速率发生，其速率接近或接近天然流程。此外，观察到光引发的DNA压缩，没有剪切或混合的影响，提供了在大分子末端成核的缩合机制的证据。用于了解光表面活性剂与DNA相互作用方式的溴化乙锭置换研究还揭示了静电力和疏水力在表面活性剂结合和DNA缩合中的重要性。

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