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首页> 外文期刊>Journal of Plant Physiology >Fulvic acid affects proliferation and maturation phases in Abies cephalonica embryogenic cells.
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Fulvic acid affects proliferation and maturation phases in Abies cephalonica embryogenic cells.

机译：黄腐酸会影响头穗冷杉（Abies cephalonica）胚发生细胞的增殖和成熟阶段。

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摘要

Embryogenic cell masses (ECM) of Abies cephalonica were grown on proliferation media in the presence and absence of fulvic acid (FA), whose molecular composition and conformational rigidity were evaluated by CPMAS-13C NMR spectroscopy. To assess the physiological effects of this humic material during proliferation and maturation stages of somatic embryogenesis (SE), proliferation rate, proportion of consecutive developmental stages of pro-embryogenic masses (PEM), cellular ATP and glucose-6-phosphate were evaluated at regular intervals. FA increased the proliferation rate, especially during the early sampling days, and the percentage of PEM in their advanced developmental stage. Cellular ATP and glucose-6-phospahte were increased by FA pre-treatment during the maturation phase. Furthermore, the effects of the anti-auxin p-chlorophenoxyisobutyric acid (PCIB), such as a decrease of growth and the enhancement of PEM III induction, were inverted by FA. Proton pumping ATPase and PPase activities were decreased in microsomes from PCIB-treated ECM, while they increased in the presence of FA. This fulvic matter also induced a delay in somatic embryo formation during the maturation phase. Both the improvement of the PEM proliferation and the reduction of the subsequent maturation process of A. cephalonica are explained by a release from the complex humic structure of low molecular-weight molecules, which may interact with the plant hormonal signaling pathway. These effects appear to be related to the hydrophilic and conformationally labile nature of FA. The structure-activity relationship observed here suggests that the influence of FA on ECM may be attributed to specific bioactive molecules that are preferentially released from the FA loose superstructure.
机译：在存在和不存在黄腐酸（FA）的情况下，将 Abies cephalonica 的胚发生细胞团（ECM）生长在增殖培养基上，并通过CPMAS- 13评估其分子组成和构象刚度。 sup C NMR光谱。为了评估这种腐殖质在体细胞胚发生（SE）的增殖和成熟阶段的生理效应，定期评估增殖速率，前胚发生质量（PEM），细胞ATP和葡萄糖6-磷酸葡萄糖的连续发育阶段所占的比例间隔。 FA增加了增殖率，尤其是在早期采样日，并提高了PEM处于晚期发育阶段的百分比。在成熟阶段，FA预处理可增加细胞的ATP和葡萄糖6磷酸。此外，FA逆转了抗生长素对氯苯氧基异丁酸（PCIB）的作用，例如生长减少和PEM III诱导增强。在PCIB处理的ECM中，微粒体中质子泵的ATPase和PPase活性降低，而在FA的存在下它们增加。该黄腐物质还诱导了成熟阶段的体细胞胚形成的延迟。 PEM增殖的改善和随后iA的成熟过程的减少。低分子量分子从复杂的腐殖质结构中释放可能解释了头颅病，这可能与植物激素信号通路相互作用。这些作用似乎与FA的亲水性和构象不稳定性质有关。此处观察到的构效关系表明，FA对ECM的影响可能归因于特定的生物活性分子，这些分子优先从FA松散的上层建筑中释放出来。

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