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首页> 外文期刊>Journal of Plant Physiology >Differences in leaf proteome response to cold acclimation between Lolium perenne plants with distinct levels of frost tolerance.
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Differences in leaf proteome response to cold acclimation between Lolium perenne plants with distinct levels of frost tolerance.

机译：耐寒性不同的黑麦草植物叶片蛋白质组对冷驯化反应的差异。

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摘要

Perennial ryegrass (Lolium perenne) is a high quality forage and turf grass mainly due to its excellent nutritive values and rapid establishment rate. However, this species has limited ability to perform in harsh winter climates. Though winter hardiness is a complex trait, it is commonly agreed that frost tolerance (FT) is its main component. Species growing in temperate regions can acquire FT through exposure to low, non-lethal temperatures, a phenomenon known as cold acclimation (CA). The research on molecular basis of FT has been performed on the model plants, but they are not well adapted to extreme winter climates. Thus, the mechanisms of cell response to low temperature in winter crops and agronomically important perennial grasses have yet to be revealed. Here, two L. perenne plants with contrasting levels of FT, high frost tolerant (HFT) and low frost tolerant (LFT) plants, were selected for comparative proteomic research. The work focused on analyses of leaf protein accumulation before and after 2, 8, 26 h, and 3, 5, 7, 14 and 21 days of CA, using a high-throughput two-dimensional electrophoresis, and on the identification of proteins which were accumulated differentially between the selected plants by the application of mass spectrometry (MS). Analyses of 580 protein profiles revealed a total of 42 (7.2%) spots that showed at a minimum of 1.5-fold differences in protein abundance, at a minimum of at one time point of CA between HFT and LFT genotypes. It was shown that significant differences in profiles of protein accumulation between the analyzed plants appeared most often on the 5th (18 proteins) and the 7th (19 proteins) day of CA. The proteins derived from 35 (83.3%) spots were successfully identified by the use of MS and chloroplast proteins were shown to be the major group selected as differentially accumulated during CA. The functions of the identified proteins and their probable influence on the level of FT in L. perenne are discussed.
机译：多年生黑麦草（ Lolium perenne ）是高质量的草料和草皮草，主要是由于其优异的营养价值和快速的定植率。但是，该物种在严酷的冬季气候下的表现能力有限。尽管耐寒性是一个复杂的特征，但人们普遍认为，耐霜性（FT）是其主要成分。在温带地区生长的物种可以通过暴露于非致命的低温环境中获得FT，这种现象称为冷驯化（CA）。 FT的分子基础研究已在模型植物上进行，但它们不适用于极端冬季气候。因此，尚未揭示冬季作物和农学上重要的多年生禾草对低温的细胞响应机制。在这里，两个L。选择了具有对比水平的FT，高耐霜性（HFT）和低耐霜性（LFT）的perenne植物进行蛋白质组研究。该工作重点在于使用高通量二维电泳分析CA的2、8、26 h和3、5、7、14和21天之前和之后的叶片蛋白质积累，并利用蛋白质进行鉴定。通过质谱分析法（MS）在选定的植物之间差异积累。对580种蛋白质谱的分析显示，共有42个（7.2％）点在HFT和LFT基因型之间的CA的一个时间点显示出至少1.5倍的蛋白质丰度差异。结果表明，被分析植物之间蛋白质积累特征的显着差异最经常出现在CA的第5天（18个蛋白质）和第7天（19个蛋白质）。通过使用质谱法成功鉴定了来自35个斑点（83.3％）的蛋白质，叶绿体蛋白质被证明是在CA期间差异积累的主要组。鉴定出的蛋白质的功能及其对FT中FT水平的可能影响。佩雷纳（Perenne）。

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