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Short term signaling responses in roots of young soybean seedlings exposed to cadmium stress

机译:镉胁迫下大豆幼苗幼苗根系的短期信号响应

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摘要

In the present study, the expression of fourteen genes involved in various signal transduction pathways was examined in young soybean (Glycine max) seedlings exposed to cadmium at two concentrations (10 mg L-1 and 25 mg L-1) for short time periods (3, 6 and 24 h). The results show that cadmium causes induction of genes encoding proteins involved in ethylene and polyamines metabolism, nitric oxide generation, MAPK cascades and regulation of other genes' expression. The bioinformatic analysis of promoter sequences of Cd-inducible genes revealed that their promoters possess several regulative motifs associated with the plant response to stress factors and abscisic acid and ethylene signaling. The involvement of ethylene in the response of soybean seedlings to cadmium stress was further confirmed by the realtime analysis of ethylene production during 24 h of CdCl2 treatment. The role of the described signaling elements in transduction of the cadmium signal in young soybean seedlings is discussed
机译:在本研究中,在短时间内暴露于两种浓度(10 mg L-1和25 mg L-1)的镉暴露的大豆(Glycine max)幼苗中,检查了14条基因参与各种信号转导途径的表达( 3、6和24小时)。结果表明,镉可诱导编码与乙烯和多胺代谢,一氧化氮生成,MAPK级联和其他基因表达调控有关的蛋白质的基因。 Cd诱导型基因启动子序列的生物信息学分析表明,它们的启动子具有与植物对逆境因子,脱落酸和乙烯信号的响应相关的几个调控基序。通过对CdCl2处理24小时内乙烯产量的实时分析,进一步证实了乙烯参与大豆幼苗对镉胁迫的反应。讨论了所述信号转导元素在大豆幼苗幼苗中镉信号转导中的作用。

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