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首页> 外文期刊>Journal of Plant Physiology >The tomato phosphatidylinositol-phospholipase C2 ( SlPLC2) is required for defense gene induction by the fungal elicitor xylanase.
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The tomato phosphatidylinositol-phospholipase C2 ( SlPLC2) is required for defense gene induction by the fungal elicitor xylanase.

机译:真菌激发子木聚糖酶诱导防御基因需要番茄磷脂酰肌醇-磷脂酶C2(SlPLC2)。

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摘要

The tomato [ Solanum lycopersicum ( Sl)] phosphatidylinositol-phospholipase C ( PI-PLC) gene family is composed of six members, named SlPLC1 to SlPLC6, differentially regulated upon pathogen attack. We have previously shown that the fungal elicitor xylanase rapidly induces nitric oxide (NO), which is required for PI-PLCs activity and downstream defense responses in tomato cell suspensions. Here, we show that all six SlPLC genes are expressed in tomato cell suspensions. Treatment of the cells with xylanase induces an early increase in SlPLC5 transcript levels, followed by a raise of the amount of SlPLC2 transcripts. The production of NO is required to augment SlPLC5 transcript levels in xylanase-treated tomato cells. Xylanase also induces SlPLC2 and SlPLC5 transcript levels in planta. We knocked-down the expression of SlPLC2 and SlPLC5 by virus-induced gene silencing. We found that SlPLC2 is required for xylanase-induced expression of the defense-related genes PR1 and HSR203J.
机译:番茄[Solaum lycopersicum(Sl)]磷脂酰肌醇-磷脂酶C(PI-PLC)基因家族由六个成员组成,分别命名为SlPLC1至SlPLC6,在病原体侵袭时受到差异调节。先前我们已经表明,真菌引发剂木聚糖酶可快速诱导一氧化氮(NO),这是PI-PLCs活性和番茄细胞悬液中下游防御反应所必需的。在这里,我们显示了所有六个SlPLC基因均在番茄细胞悬液中表达。用木聚糖酶处理细胞诱导SlPLC5转录物水平的早期增加,然后增加SlPLC2转录物的量。需要NO的产生以增加木聚糖酶处理的番茄细胞中SlPLC5转录物水平。木聚糖酶还诱导植物中SlPLC2和SlPLC5的转录水平。我们通过病毒诱导的基因沉默敲低了SlPLC2和SlPLC5的表达。我们发现木聚糖酶诱导的防御相关基因PR1和HSR203J的表达需要SlPLC2。

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