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首页> 外文期刊>Journal of Plant Physiology >Multistep involvement of glutathione with salicylic acid and ethylene to combat environmental stress.
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Multistep involvement of glutathione with salicylic acid and ethylene to combat environmental stress.

机译：谷胱甘肽与水杨酸和乙烯的多步反应可消除环境压力。

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摘要

The role of glutathione (GSH) in plant defense is an established fact. However, the association of GSH with other established signaling molecules within the defense signaling network remains to be evaluated. Previously we have shown that GSH is involved in defense signaling network likely through NPR1-dependent salicylic acid (SA)-mediated pathway. In this study, to gain further insight, we developed chloroplast-targeted gamma-glutamylcysteine synthetase (gamma-ECS) overexpressed transgenic Nicotiana tabacum ( NtGp line) and constructed a forward subtracted cDNA (suppression subtractive hybridization (SSH)) library using NtGp line as a tester. Interestingly, in addition to SA-related transcripts like pathogenesis-related protein 1a ( PR1a) and SAR8.2 m/2 l, 1- aminocyclopropane-1- carboxylate oxidase ( ACC oxidase), a key enzyme of ethylene (ET) biosynthesis, was identified in the SSH library. Besides, transcription factors like WRKY transcription factor 3 ( WRKY3), WRKY1 and ethylene responsive factor 4 ( ERF4), associated with SA and ET respectively, were also identified thus suggesting an interplay of GSH with ET and SA. Furthermore, proteomic profiling of NtGp line, performed by employing two-dimensional gel electrophoresis (2-DE), corroborated with the transcriptomic profile and several defense-related proteins like serine/threonine protein kinase, and heat shock 70 protein (HSP70) were identified with increased accumulation. Fascinatingly, induction of 1-aminocyclopropane-1-carboxylate synthase (ACC synthase) was also noted thus demonstrating the active involvement of GSH with ET. Protein gel blot analysis confirmed the enhanced accumulation of ACC oxidase in NtGp line. Together, our data revealed that GSH is involved in the synergistic multiple steps crosstalk through ET as well as SA to combat environmental stress.
机译：谷胱甘肽（GSH）在植物防御中的作用已是事实。然而，GSH与防御信号网络内其他已建立的信号分子的关联仍有待评估。以前我们已经表明，GSH可能通过依赖NPR1的水杨酸（SA）介导的途径参与了防御信号网络。在这项研究中，为了获得更多的见识，我们开发了过表达转基因烟草的叶绿体靶向的γ-谷氨酰半胱氨酸合成酶（γ-ECS），并使用NtGp系构建了正向扣除的cDNA（抑制消减杂交（SSH））文库测试员。有趣的是，除了与病原相关的蛋白1a（PR1a）和SAR8.2 m / 2 l等SA相关的转录本外，乙烯生物合成的关键酶1-氨基环丙烷-1-甲酸氧化酶（ACC氧化酶），在SSH库中被识别。此外，还确定了分别与SA和ET相关的WRKY转录因子3（WRKY3），WRKY1和乙烯响应因子4（ERF4）等转录因子，表明GSH与ET和SA相互作用。此外，通过二维凝胶电泳（2-DE）对NtGp株进行了蛋白质组学分析，并证实了转录组学特征，并鉴定了一些防御相关蛋白，如丝氨酸/苏氨酸蛋白激酶和热休克70蛋白（HSP70）。随着积累的增加。令人着迷的是，还注意到了1-氨基环丙烷-1-羧酸合酶（ACC合酶）的诱导，从而证明了GSH参与了ET。蛋白质凝胶印迹分析证实NtGp系中ACC氧化酶的积累增强。总之，我们的数据表明GSH通过ET和SA参与了多重多重串扰，以对抗环境压力。

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