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首页> 外文期刊>Journal of Plant Physiology >Coronatine alleviates salinity stress in cotton by improving the antioxidative defense system and radical-scavenging activity
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Coronatine alleviates salinity stress in cotton by improving the antioxidative defense system and radical-scavenging activity

机译:日冕碱通过改善抗氧化防御系统和清除自由基的活性来减轻棉花的盐分胁迫

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摘要

Coronatine (COR) is a chlorosis-inducing phytotoxin that mimics some biological activities of methyl jasmonate. This study investigated whether COR confers salinity tolerance to cotton and whether such tolerance is correlated with changes in the activity of antioxidant enzymes. COR at 0.01muM was applied hydroponically to cotton seedlings at the two-leaf stage for 24h. A salinity stress of 150mM NaCl was imposed after completion of COR treatment for 15d. Salinity stress reduced biomass of seedlings and increased leaf superoxide radicals, hydrogen peroxide, lipid peroxidation, and electrolyte leakage. Activities of the antioxidant enzymes superoxide dismutase (SOD), catalase (CAT), peroxidase (POD), and glutathione reductase (GR), and of the stable free radical, 1,1-diphenyl-2-picrylhydrazyl (DPPH), scavenging activity were altered by salinity to varying degrees. Pretreatment with COR increased the activities of CAT, POD, GR, and DPPH scavenging activity in leaf tissues of salinity-stressed seedlings. Thus, COR might reduce the production of reactive oxygen species by activating antioxidant enzymes and DPPH-radical scavenging, thereby preventing membrane peroxidation and denaturation of bio-molecules.
机译:Coronatine(COR)是一种诱导绿化的植物毒素,模仿茉莉酸甲酯的某些生物活性。这项研究调查了COR是否赋予棉花耐盐性,以及这种耐性是否与抗氧化酶活性的变化相关。在两叶阶段,将0.01μM的COR水培施用于棉花幼苗,持续24h。 COR处理15天后,施加150mM NaCl的盐度应力。盐分胁迫减少了幼苗的生物量,并增加了叶片超氧自由基,过氧化氢,脂质过氧化和电解质泄漏。抗氧化酶超氧化物歧化酶(SOD),过氧化氢酶(CAT),过氧化物酶(POD)和谷胱甘肽还原酶(GR)的活性以及稳定的自由基1,1,1-二苯基-2-吡啶并肼基(DPPH)的清除活性被盐度不同程度地改变了。用COR预处理可提高盐分胁迫幼苗叶片组织中CAT,POD,GR和DPPH的清除活性。因此,COR可能会通过激活抗氧化酶和DP​​PH自由基来减少活性氧的产生,从而防止膜的过氧化和生物分子的变性。

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