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首页> 外文期刊>Journal of Plant Physiology >Involvement of non-phosphorylating glyceraldehyde-3-phosphate dehydrogenase in response to oxidative stress
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Involvement of non-phosphorylating glyceraldehyde-3-phosphate dehydrogenase in response to oxidative stress

机译：非磷酸化3-磷酸甘油醛脱氢酶参与氧化应激

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摘要

Glyceraldehyde-3-phosphate dehydrogenases catalyze key steps in energy and reducing power partitioning in cells of higher plants. Because non-phosphorylating glyceraldehyde-3-phosphate dehydrogenase (NP-Ga3PDHase) is involved in the production of reductive power (NADPH) in the cytosol, its behavior under oxidative stress conditions was analyzed. The specific activity of the enzyme was found to increase up to 2-fold after oxidative conditions imposed by methylviologen in wheat and maize seedlings. Under moderate oxidant concentration, lack of mRNA induction was observed. The increase in specific activity would thus be a consequence of a significant stability of NP-Ga3PDHase. Our results suggest that the enzyme could be modified by oxidation of cysteine residues, but formation of disulfide bridges is dependent on levels of divalent cations and 14-3-3 proteins. The latter differential effect could be critical to relatively maintain energy and reductant levels in the cytoplasm of plant cells under oxidative stress.
机译：3-磷酸​​甘油醛脱氢酶催化能量的关键步骤并减少高等植物细胞中的功率分配。由于非磷酸化3-磷酸甘油醛脱氢酶（NP-Ga3PDHase）参与了细胞质溶胶中还原能力（NADPH）的产生，因此分析了其在氧化应激条件下的行为。在小麦和玉米幼苗中，甲基紫精施加氧化条件后，发现该酶的比活性增加了两倍。在中等氧化剂浓度下，观察到缺乏mRNA诱导。因此，比活性的增加是NP-Ga3PDHase的显着稳定性的结果。我们的结果表明，可以通过氧化半胱氨酸残基来修饰该酶，但是二硫键的形成取决于二价阳离子和14-3-3蛋白的水平。后者的差异作用对于在氧化胁迫下相对维持植物细胞的细胞质中的能量和还原剂水平可能至关重要。

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