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首页> 外文期刊>Journal of Plant Physiology >Sodium induces simultaneous changes in cytosolic calcium and pH in salt-tolerant quince protoplasts
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Sodium induces simultaneous changes in cytosolic calcium and pH in salt-tolerant quince protoplasts

机译：钠诱导耐盐木瓜原生质体中胞浆钙和pH的同时变化

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Previous experiments with salt-resistant quince BA29 (Cydonia oblonga cv. Mill.) have shown that this cultivar takes up sodium transiently into the cytosol of shoot protoplasts only in the absence of calcium chloride, or at <1 mM calcium chloride. Addition of NaCl E100 mM to single protoplasts from in vitro-cultivated quince in the presence of 1.0 mM calcium induced instant changes in the cytosolic concentrations of calcium and protons. These changes were investigated by use of tetra [acetoxymethyl] esters of the fluorescent stilbene chromophores Fura 2 and bis-carboxyethyl-carboxyfluorescein (BCECF), respectively. The cytosolic Ca2+ dynamics in the protoplasts were dependent on the concentration of NaCl added. The changes in calcium differed in amplitude and final concentration and were correlated in time mainly with changes in pH. Addition of 100400 mM NaCl to the protoplasts caused an oscillating increase in the cytosolic level of calcium, and then a decrease. Addition of mannitol, of equiosmolar concentration to NaCl, did not increase the cytosolic calcium concentration. Moreover, there was no increase in cytosolic calcium when NaCl was added in the presence of calcium binding ethylene glycol-bis(beta-aminoethylether)-N,N,No,No-tetra acetic acid (EGTA), or lantan or verapamil, two inhibitors of plasma membrane calcium channels. Therefore, we conclude that, in salt-resistant quince, sodium induces an influx of calcium into the cytosol by plasma membrane calcium channels, and a simultaneous increase in cytosolic pH. Because these changes were obtained in the presence of 1 mM calcium in the medium, they were not due to sodium uptake into the cytosol.
机译：以前用耐盐木瓜BA29（长圆Cydenonia cv。Mill。）进行的实验表明，该品种仅在不存在氯化钙或<1 mM氯化钙的情况下，才将钠瞬时吸收到芽原生质体的细胞质中。在存在1.0 mM钙的情况下，将NaCl E100 mM加到来自体外培养的木瓜的单个原生质体中，可以诱导钙和质子的胞质浓度即时变化。通过分别使用荧光二苯乙烯生色团Fura 2和双羧乙基-羧基荧光素（BCECF）的四[乙酰氧基甲基]酯研究了这些变化。原生质体中的胞质Ca2 +动态取决于所添加的NaCl浓度。钙的变化幅度和最终浓度不同，并且与时间的变化主要与pH的变化相关。向原生质体中添加100400 mM NaCl会引起钙的胞浆水平振荡上升，然后下降。将等摩尔浓度的甘露醇添加到NaCl中，不会增加胞质钙的浓度。此外，当在钙结合的乙二醇-双（β-氨基乙基醚）-N，N，No，No-四乙酸（EGTA）或lantan或verapamil两种存在钙的情况下添加NaCl时，胞质钙没有增加。质膜钙通道的抑制剂。因此，我们得出结论，在耐盐的木瓜中，钠通过质膜钙通道诱导钙流入胞质溶胶，并同时增加胞质pH值。因为这些变化是在培养基中存在1 mM钙的情况下获得的，所以它们并不是由于钠吸收到胞质溶胶中引起的。

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