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首页> 外文期刊>Journal of Plant Physiology >Enhancement of the differentiation of protocorm-like bodies of Dendrobium officinale to shoots by ultrasound treatment.
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Enhancement of the differentiation of protocorm-like bodies of Dendrobium officinale to shoots by ultrasound treatment.

机译：超声波处理增强铁皮石D原球茎样体向芽的分化。

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摘要

An efficient micropropagation protocol has been developed for Dendrobium officinale, through protocorm-like bodies (PLBs). A correlation between enhanced differentiation of PLBs of D. officinale by ultrasound and changes in the levels of endogenous hormones and the antioxidant enzyme activities was described. Ultrasound treatments improved the conversion of PLBs of D. officinale to shoots. The highest conversion frequency of PLBs to shoots was obtained following the ultrasound treatment at 300 W for 5 min. Compared to the control, the enhanced conversion of PLBs to shoots following the ultrasound treatment was accompanied by an increase in the ratio of total cytokinins (CTKs) to indole-3-acetic acid (IAA), which was due to a decrease in the endogenous level of IAA and an increase in the endogenous level of total CTKs. Analysis of enzyme activities indicated that the increased endogenous level of total CTKs driven by ultrasound was associated with the inhibition of CTK decomposition by CTK oxidase (EC 1.4.3.6), while the decreased endogenous level of IAA was associated with the promotion of IAA decomposition by IAA oxidase (EC 1.10.3.3). In addition, ultrasound treatment increased the activities of superoxide dismutase (EC 1.15.1.1), catalase (EC 1.11.1.6) and peroxidase (EC 1.11.1.7) in the conversion process of PLBs to shoots.
机译：已经通过原球茎样体（PLB）为石D（Dendrobium officinale）开发了一种有效的微繁殖方案。 iD的PLB的增强分化之间的相关性。描述了通过超声波处理药材，以及内源激素水平和抗氧化酶活性的变化。超声处理提高了DPL的转化率。来发芽。在300 W超声处理5分钟后，PLBs转化为芽的最高频率。与对照相比，超声处理后PLB向芽的转化增强，同时总细胞分裂素（CTK）与吲哚-3-乙酸（IAA）的比率增加，这是由于内源性物质的减少IAA的水平以及总CTK的内源性水平的增加。酶活性分析表明，超声驱动的总CTK内源性水平升高与CTK氧化酶抑制CTK分解有关（EC 1.4.3.6），而IAA的内源性水平降低与IAA促进内毒素分解有关。 IAA氧化酶（EC 1.10.3.3）。此外，超声波处理增加了PLB转化为芽的过程中超氧化物歧化酶（EC 1.15.1.1），过氧化氢酶（EC 1.11.1.6）和过氧化物酶（EC 1.11.1.7）的活性。

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