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首页> 外文期刊>Journal of Plant Physiology >The presence of soluble carbonic anhydrase in the thylakoid lumen of chloroplasts from Arabidopsis leaves.
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The presence of soluble carbonic anhydrase in the thylakoid lumen of chloroplasts from Arabidopsis leaves.

机译：拟南芥叶片叶绿体类囊体腔中可溶性碳酸酐酶的存在。

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摘要

Supernatant obtained after high-speed centrifugation of disrupted thylakoids that had been washed free from extrathylakoid carbonic anhydrases demonstrated carbonic anhydrase activity that was inhibited by the specific inhibitors acetazolamide and ethoxyzolamide. A distinctive feature of the effect of Triton X-100 on this activity also suggested that the source of the activity is a soluble protein. Native electrophoresis of a preparation obtained using chromatography with agarose/mafenide as an affinity sorbent revealed one protein band with carbonic anhydrase activity. The same protein was revealed in a mutant deficient in soluble stromal carbonic anhydrase beta-CA1, and this indicated that the newly revealed carbonic anhydrase is not a product of the At3 g01500 gene. These data imply the presence of soluble carbonic anhydrase in the thylakoid lumen of higher plants.
机译：高速离心分离的类囊体并离心分离后得到的上清液，该类囊体已从类囊外碳酸酐酶中洗去，显示出碳酸酐酶活性受到特定抑制剂乙酰唑胺和乙氧基唑酰胺的抑制。 Triton X-100对该活性作用的显着特征还表明，该活性的来源是可溶性蛋白。使用琼脂糖/马芬尼作为亲和吸附剂的色谱法得到的制剂的天然电泳显示出一个具有碳酸酐酶活性的蛋白带。在缺乏可溶性间质碳酸酐酶β-CA1的突变体中发现了相同的蛋白质，这表明新发现的碳酸酐酶不是At3 g01500基因的产物。这些数据表明高等植物类囊体腔中存在可溶性碳酸酐酶。

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