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Data reduction methods for ektacytometry in clinical hemorheology

机译：血液流式细胞仪在临床血液流变学中的数据缩减方法

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Laser-diffraction ektacytometry is a generally accepted technique for measuring RBC deformability induced by fluid shear stress (SS) and yields paired elongation index-SS data at several levels of stress. Unfortunately, comparison of results is hindered by the lack of simple indices that accurately characterize these data. Several mathematical models have been proposed, including those developed for analysis of enzyme kinetics (Lineweaver-Burk, Eadie-Hofstee) and curve fitting (Streekstra-Bronkhorst). All of these analytical approaches provide a value for cell deformation at infinite stress (EImax) and the shear stress required to achieve one-half of this deformation (SS1/2); the use of non-linear regression is essential when calculating these parameters. While the current models provide equivalent results for normal RBC if used with non-linear regression, EImax and SS1/2 are not always concordant for cells with abnormal mechanical behavior. This technical note examines such differences for three conditions: glutaraldehyde treatment, mechanical stress and non-isotonic media. It was found that none of the models yield completely satisfactory values for EI max and SS1/2, especially if there are large changes of EImax. However, the ratio of SS1/2 to EImax (SS1/2/EImax) is much less affected by these problems, has similar power (i.e., standardized difference) as SS1/2 and EI max and is more robust in reflecting alterations of deformability. We thus conclude that the SS1/2/EImax ratio can be used when reporting and comparing various populations of RBC or cells obtained from subjects having different clinical states.
机译：激光衍射流式细胞仪是一种用于测量由流体剪切应力（SS）引起的RBC变形能力的普遍接受的技术，并在多个应力水平下产生成对的伸长指数-SS数据。不幸的是，结果的比较由于缺少能够准确表征这些数据的简单索引而受到阻碍。已经提出了几种数学模型，包括为分析酶动力学而开发的数学模型（Lineweaver-Burk，Eadie-Hofstee）和曲线拟合（Streekstra-Bronkhorst）。所有这些分析方法都可提供无限应力（EImax）下的单元变形值和达到该变形一半的所需剪应力（SS1 / 2）值。计算这些参数时，必须使用非线性回归。如果当前模型与非线性回归一起使用时，可以为正常RBC提供等效的结果，但对于具有异常机械行为的细胞，EImax和SS1 / 2并不总是一致的。本技术说明研究了三种条件下的这种差异：戊二醛处理，机械应力和非等渗介质。结果发现，没有一个模型能够得出完全令人满意的EI max和SS1 / 2值，特别是当EI max发生较大变化时。但是，SS1 / 2与EImax的比率（SS1 / 2 / EImax）受这些问题的影响要小得多，具有与SS1 / 2和EImax相似的功效（即，标准差），并且在反映变形能力的变化方面更强大。因此，我们得出结论，当报告和比较从具有不同临床状态的受试者获得的红细胞或细胞的各种种群时，可以使用SS1 / 2 / EImax比值。

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