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首页> 外文期刊>Biophysical Chemistry: An International Journal Devoted to the Physical Chemistry of Biological Phenomena >Raman micro-spectroscopy: A powerful tool for the monitoring of dynamic supramolecular changes in living cells
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Raman micro-spectroscopy: A powerful tool for the monitoring of dynamic supramolecular changes in living cells

机译：拉曼光谱：监测活细胞动态超分子变化的强大工具

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摘要

Cellular imaging techniques have become powerful tools in cell biology. With respect to others, the techniques based on vibrational spectroscopy present a clear advantage: the molecular composition and the modification of subcellu-lar compartments can be obtained in label-free conditions. In fact, from the evolution of positions, intensities and line widths of Raman and infrared bands in the cell spectra, characteristic information on cellular activities can be achieved, and particularly, cellular death can be investigated. In this work we present the time evolution of the Raman spectra of single live Jurkat cells (T-lymphocyte) by looking at the high frequency part of their Raman spectra, that is the CH stretching region, around 3000 cm"1. In particular, investigation into the composition or rearrangement of CH bounds, markers of cellular membrane fatty acids, can represent an important method to study and to recognize cell death. The experimental procedure we used, together with the analysis of these high frequency vibrational bands, may represent a new, improved and advantageous approach to this kind of study.
机译：细胞成像技术已成为细胞生物学中的强大工具。关于其他方面，基于振动光谱的技术具有明显的优势：可以在无标签的条件下获得分子组成和亚细胞隔室的修饰。实际上，从细胞光谱中拉曼光谱和红外光谱带的位置，强度和线宽的演变，可以获得有关细胞活性的特征信息，特别是可以研究细胞死亡。在这项工作中，我们通过观察单个活Jurkat细胞（T淋巴细胞）的拉曼光谱的高频部分（即CH拉伸区域，大约3000 cm“ 1）来展示其拉曼光谱的时间演化。特别是， CH膜的组成或重排（细胞膜脂肪酸的标志物）的研究可能是研究和识别细胞死亡的重要方法，我们使用的实验程序以及对这些高频振动带的分析可能代表了这种研究的新的，改进的和有利的方法。

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