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首页> 外文期刊>Journal of Pathology: Journal of the Pathological Society of Great Britain and Ireland >A highly sensitive detection method for immunohistochemistry using biotinylated tyramine.
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A highly sensitive detection method for immunohistochemistry using biotinylated tyramine.

机译:使用生物素化酪胺的免疫组织化学的高灵敏度检测方法。

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摘要

A highly sensitive method for light microscopic immunohistochemistry is described. The increased sensitivity compared with current methodologies is based on the horseradish peroxidase-catalysed deposition of biotinylated tyramine at the sites of immunoreactivity, followed by the detection of the biotin with streptavidin biotin horseradish peroxidase complex. This method is of general applicability in immunohistochemistry and has several important advantages over currently used immunohistochemical detection procedures. The most significant advantage is that several antibodies which to data have been non-reactive, even in antigen-retrieval formalin-fixed, was-embedded sections, now show strong and reproducible immunoreactivity using biotinylated tyramine amplification. In addition, many other antibodies can be used at significantly higher dilutions.
机译:描述了一种用于光学显微免疫组织化学的高度敏感的方法。与当前方法相比,灵敏度的提高是基于辣根过氧化物酶催化的生物素化酪胺在免疫反应位点的沉积,然后用链霉亲和素生物素辣根过氧化物酶复合物检测生物素。该方法在免疫组织化学中具有普遍适用性,与目前使用的免疫组织化学检测程序相比具有几个重要的优点。最显着的优点是,即使在抗原回收福尔马林固定的,被包埋的切片中,几种对数据无反应的抗体现在也可以通过生物素化酪胺扩增显示出强而可再现的免疫反应性。另外,可以以更高的稀释度使用许多其他抗体。

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