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首页> 外文期刊>Journal of Pure & Applied Microbiology >Identification and Genetic Characterization of Pectobacterium spp. and Related Enterobacteriaceae Causing Potato Soft Rot Diseases in Egypt
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Identification and Genetic Characterization of Pectobacterium spp. and Related Enterobacteriaceae Causing Potato Soft Rot Diseases in Egypt

机译：副杆菌的鉴定和遗传特性。埃及马铃薯软腐病的致病菌和相关肠杆菌科

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摘要

Seventeen soft rot bacteria were isolated from potato tubers and stems snowing like symptoms of soft rot and blackleg. Initial characterization concerning morphological and biochemical characteristics was performed. Furthermore, all bacterial isolatesshowed symptoms of the soft rot disease upon infection of potato tubers with different pathogenic capability. Molecular identification of these isolates via PCR utilizing the 16S rRNA universal primers was carried out. DNA sequence of PCR products and analysis via BLAST and data of the Genbank showed that, twelve isolates belonging to Pectobacterium carotovorum sub sp. carotovorum, three isolates belonging to Enterobacter cloacae and one isolate of each P. carotovorum subsp. brasiliense and Dickeya chrysanthemi. Moreover, the P. carotovorum subsp brasiliense isolate was confirmed by PCR amplification using specific primers (Brlf/Llr) and represented the expected length 322 bp. The genetic diversity among the seventeen bacterial isolates was investigated by Random Amplified Polymorphic DNA (RAPD) analysis, using 6 different primers, revealed different levels of molecular variation among bacterial isolates on the bases of amplified product pattern. The RAPD analysis shows that isolates of P. carotovorum subspecies were quite homogenous and could be differentiated from Enterobacter. cloacae isolates.
机译：从马铃薯块茎中分离出十七种软腐细菌，茎秆下雪，出现软腐和黑腿病症状。进行了有关形态和生化特性的初步表征。此外，在感染具有不同致病能力的马铃薯块茎时，所有细菌分离株均表现出软腐病症状。使用16S rRNA通用引物通过PCR对这些分离物进行分子鉴定。 PCR产物的DNA序列和通过BLAST的分析以及Genbank的数据显示，十二种分离物属于胡萝卜杆菌亚种。 carotovorum，属于阴沟肠杆菌的三个分离株，每个P. carotovorum亚种的分离株。巴西利亚和Dickeya chrysanthemi。此外，通过使用特定引物（Brlf / Llr）的PCR扩增证实了胡萝卜假单胞菌巴西亚种的分离株，其代表预期的长度322bp。通过随机扩增多态性DNA（RAPD）分析，使用6种不同的引物，对17种细菌分离株的遗传多样性进行了研究，揭示了在细菌分离株之间基于扩增产物模式的不同水平的分子变异。 RAPD分析表明，胡萝卜假单胞菌亚种的分离物非常同源，可以与肠杆菌区别开来。泄殖腔分离株。

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