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首页> 外文期刊>Journal of Pure & Applied Microbiology >Research on Microbial Diversity of Pipeline Biofilms in Water Distribution System
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Research on Microbial Diversity of Pipeline Biofilms in Water Distribution System

机译：配水系统中管道生物膜的微生物多样性研究

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The biofilms have been described as "cities of microbes". Some studies have suggested that pathogenic bacteria, which may not be detected by culture-dependent methods, occur in the biofilms. So a pilot drinking water distribution system is constructed and the combination of the scanning electron microscope (SEM) and the polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) is used to show the microbial growth of the biofilms in the pipe wall macroscopically and microscopically. The experiment simulates the water supply network and cultivates the biofilms for 7 months, observing the growth of the biofilms in the network entrance and end intuitively by SEM. And the microbial diversity in biofilms at different seasons and with different materials is evaluated by PCR-DGGE based on 16SrDNA. The result shows that the pipe material affects the biofilm bacteria, total bacteria and bacteria diversity; the number in the galvanized steel pipe is larger; the growth of the biofilmsin the PE pipe and UPVC pipe are slowed down. The temperature is changed by the season, which is the main reason that causes the change of the diversity of bacteria. Both the hydraulic condition and residue chlorine concentration affect the growth of the biofilms in the pipe wall.
机译：生物膜被描述为“微生物之城”。一些研究表明，致病细菌可能无法通过培养依赖的方法检测到，但会在生物膜中发生。因此，构建了一个中试饮用水分配系统，并结合使用了扫描电子显微镜（SEM）和聚合酶链反应-变性梯度凝胶电泳（PCR-DGGE）来宏观地显示管壁中生物膜的微生物生长。和微观上。该实验模拟了供水网络并培养了7个月的生物膜，并通过SEM直观地观察了生物膜在网络入口和末端的生长情况。通过基于16SrDNA的PCR-DGGE对不同季节，不同材料的生物膜中的微生物多样性进行了评估。结果表明，管道材料影响生物膜细菌，细菌总数和细菌多样性。镀锌钢管的个数较大。 PE管和UPVC管中生物膜的生长速度减慢。温度随季节变化，这是引起细菌多样性变化的主要原因。水力条件和残余氯浓度都影响管壁中生物膜的生长。

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