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首页> 外文期刊>Journal of Pure & Applied Microbiology >Serological and Molecular Detection of Peanut Bud Necrosis Virus (PBNV) causing Bud Necrosis Disease in GroundnutAU Swamy, K. M. (kmswamy2986@gmail.com) Patil, M. S.
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Serological and Molecular Detection of Peanut Bud Necrosis Virus (PBNV) causing Bud Necrosis Disease in GroundnutAU Swamy, K. M. (kmswamy2986@gmail.com) Patil, M. S.

机译：在花生中引起芽坏死病的花生芽坏死病毒（PBNV）的血清学和分子检测AU Swamy，K.M.（kmswamy2986@gmail.com）Patil，M.S.

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摘要

Groundnut bud necrosis disease caused by Peanut bud necrosis virus (PBNV) is a distinct member in the genus Tospovirus of the Bunyaviridae, and is the most economically important disease affecting groundnut crop. Bud necrosis infected groundnut plant samples were collected from ten groundnut growing districts of Karnataka were subjected to Direct antigen coated-enzyme linked immunosorbent assay (DAC-ELISA) and reverse transcription-polymerase chain reaction (RT-PCR) to detect GBNV. All the samples showed positive reactions with polyclonal antibodies of GBNV in DAC-ELISA and amplification of 831 bp GBNV coat protein gene by RT-PCR with degenerate primers.C1 Swamy, K. M.; Univ Agr Sci, Dept Plant Pathol, Dharwad 580005, Karnataka, IndiaSC Science & Technology - Other Topics; Infectious Diseases; Genetics & Heredity; Agriculture
机译：花生芽坏死病毒（PBNV）引起的花生芽坏死病是Bunyaviridae Tospovirus属的独特成员，并且是影响花生作物的最经济重要的疾病。从卡纳塔克邦的十个花生种植区收集芽坏死感染的花生植物样品，然后进行直接抗原包被酶联免疫吸附测定（DAC-ELISA）和逆转录聚合酶链反应（RT-PCR）以检测GBNV。所有样品在DAC-ELISA中均显示与GBNV的多克隆抗体呈阳性反应，并用简并引物通过RT-PCR扩增了831 bp GBNV外壳蛋白基因。 Univ Agr Sci，部门植物病理学，印度卡纳塔克邦Dharwad 580005SC科学与技术-其他主题;传染性疾病;遗传与遗传;农业

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