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首页> 外文期刊>Journal of Pure & Applied Microbiology >A Novel Bacillus thuringiensis Crystal Protein Cry51Aa1 with Preferentially Anti-cancer ActivityAU Aldeewan, Ali B. Zhang, Yilei Meng, Ying Peng, Donghai Yu, Ziniu Sun, Ming Ring, Brian Z. Su, Li (lisu@hust.edu.cn)
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A Novel Bacillus thuringiensis Crystal Protein Cry51Aa1 with Preferentially Anti-cancer ActivityAU Aldeewan, Ali B. Zhang, Yilei Meng, Ying Peng, Donghai Yu, Ziniu Sun, Ming Ring, Brian Z. Su, Li (lisu@hust.edu.cn)

机译：A Novel Bacillus thuringiensis Crystal Protein Cry51Aa1 with Preferentially Anti-cancer ActivityAU Aldeewan, Ali B. Zhang, Yilei Meng, Ying Peng, Donghai Yu, Ziniu Sun, Ming Ring, Brian Z. Su, Li (lisu@hust.edu.cn)

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摘要

Cry51Aa1, a novel crystal protein comprised of 309 amino acid residues with a predicted molecular weight of 34 kDa, was purified from Bacillus thuringiensis strain F14-1. After trypsin digestion, we observed three smaller bands of Cry51Aa1 protein upon separation by gel. In addition, this digested Cry51Aal protein had no obvious toxicity to several types of insect larvae and hemolytic activity human erythrocyte. However, the activated Cry51Aa1 could exhibit cytocydal activity against to MDA-MB-231, HCT116, CaCo2 and U87 tumor cells, while no cytotoxic activity was found in Cry51Aa1 treated PC3, HepG2 and LO2 cells. Importantly, MDA-MB-231 cells showed the most sensitivity to activated Cry51Aa1 within all the cell lines assessed. These observations referred to the presence of a new non insecticidal toxin of Cry51Aa1, which should be a new parasporin protein.C1 Su, Li; Huazhong Univ Sci and Technol, Sch Life Sci and Technol, Minist Educ, Key Lab Mol Biophys, Wuhan 430074, Peoples R ChinaSC Biochemistry & Molecular Biology; Oncology; Pharmacology & Pharmacy
机译：从苏云金芽胞杆菌菌株F14-1中纯化了Cry51Aa1，它是一种由309个氨基酸残基组成的新型晶体蛋白，预计分子量为34 kDa。胰蛋白酶消化后，我们通过凝胶分离观察到三个较小的Cry51Aa1蛋白条带。另外，这种消化的Cry51Aal蛋白对几种类型的昆虫幼虫和具有溶血活性的人红细胞没有明显的毒性。然而，活化的Cry51Aa1可能对MDA-MB-231，HCT116，CaCo2和U87肿瘤细胞具有杀细胞活性，而在Cry51Aa1处理的PC3，HepG2和LO2细胞中未发现细胞毒性。重要的是，MDA-MB-231细胞在所有评估的细胞系中对活化的Cry51Aa1表现出最高的敏感性。这些观察结果表明存在新的Cry51Aa1非杀虫毒素，该毒素应该是新的副孢菌素蛋白。华中大学科技学院，生命科学与技术学院，教育部，分子生物学重点实验室，武汉430074，人民中国生物化学与分子生物学;肿瘤学药理与药学

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