...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of proteome research >Discovery of Manassantin A Protein Targets Using Large-Scale Protein Folding and Stability Measurements
【24h】

Discovery of Manassantin A Protein Targets Using Large-Scale Protein Folding and Stability Measurements

机译：使用大规模蛋白质折叠和稳定性测量发现Manassantin A蛋白质靶标

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Manassantin A is a natural product that has been shown to have anticancer activity in cell-based assays, but has a largely unknown mode-of action. Described here is the use of two different energetics-based approaches to identify protein targets of manassantin A. Using the stability of proteins from rates of oxidation technique with an isobaric mass tagging strategy (iTRAQ- SPROX) and the pulse proteolysis technique with a stable isotope labeling with amino acids in cell culture strategy (SILAC-PP), over 1000 proteins in a MDA-MB-231 cell lysate grown under hypoxic conditions were assayed for manassantin A interactions (both direct and indirect). A total of 28 protein hits were identified with manassantin. A-induced thermodynamic stability changes. Two of the protein hits (filamin A and elongation factor la) were identified using both experimental approaches. The remaining 26 hit proteins were only assayed in either the iTRAQ-SPROX or the SILAC-PP experiment. The 28 potential protein targets of manassantin A identified here provide new experimental avenues along which to explore the molecular basis of manassantin A's mode of action. The current work also represents the first application iTRAQ-SPROX and SILAC-PP to the large-scale analysis of protein ligand binding interactions involving a potential anticancer drug with an unknown mode-of-action.
机译：Manassantin A是一种天然产物，已在基于细胞的测定法中显示具有抗癌活性，但作用模式却非常未知。在此描述的是使用两种不同的基于能量学的方法来鉴定甘露素A的蛋白质靶标。利用氧化速率的蛋白质稳定性和等压质量标记策略（iTRAQ-SPROX）以及具有稳定同位素的脉冲蛋白水解技术在细胞培养策略（SILAC-PP）中用氨基酸标记，在缺氧条件下生长的MDA-MB-231细胞裂解液中的1000多种蛋白进行了Manassantin A相互作用（直接和间接）的分析。用manassantin鉴定出总共28个蛋白质命中。 A引起的热力学稳定性改变。使用两种实验方法都可以鉴定出两种蛋白质命中值（丝氨酸A和伸长因子1a）。剩下的26种命中蛋白只能在iTRAQ-SPROX或SILAC-PP实验中进行测定。此处确定的28种潜在的Manassantin A蛋白靶标为探索Manassantin A作用方式的分子基础提供了新的实验途径。当前的工作还代表了iTRAQ-SPROX和SILAC-PP在涉及潜在的抗癌药物且作用方式未知的蛋白质配体结合相互作用的大规模分析中的首次应用。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号