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首页> 外文期刊>Journal of proteome research >Composition and antimicrobial activity of the skin peptidome of russian brown frog rana temporaria
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Composition and antimicrobial activity of the skin peptidome of russian brown frog rana temporaria

机译：俄罗斯棕蛙蛙皮的皮肤肽组的组成及抑菌活性

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A nano-HPLC-ESI-OrbiTrap study involving HCD and ETD spectra has been carried out to clarify the composition of the skin peptidome of brown Russian frogs Rana temporaria. This approach allowed determinantion of 76 individual peptides, increasing 3-fold the identified portion of the peptidome in comparison to that obtained earlier with FTICR MS. A search for the new bradykinin related peptides (BRPs) was carried out by reconstructing mass chromatograms based on the ion current of characteristic b- and y-ions. Several peptides were reported in the secretion of R. temporaria for the first time. The overall antibacterial activity of the skin secretion in general and of one individual peptide (Brevinin 1Tb) was determined using PMEU Spectrion (Portable Microbe Enrichment Unit) technology. The inhibitory effects of these peptides on Staphylococcus aureus and Salmonella enterica Serovar typhimutium were equal in scale to that reported for some antibiotics.
机译：进行了涉及HCD和ETD光谱的纳米HPLC-ESI-OrbiTrap研究，以阐明棕色俄罗斯蛙蛙皮的皮肤肽组的组成。这种方法可以确定76个单独的肽段，与之前使用FTICR MS获得的肽段相比，肽段的鉴定部分增加了3倍。通过基于特征性b离子和y离子的离子流重建质谱图，对新的缓激肽相关肽（BRP）进行了搜索。首次报道了在R. temporaria分泌物中有几种肽。使用PMEU Spectrion（便携式微生物富集单元）技术确定皮肤分泌物的总体抗菌活性和单个肽（Brevinin 1Tb）的总体抗菌活性。这些肽对金黄色葡萄球菌和肠炎沙门氏菌鼠伤寒沙门氏菌的抑制作用在规模上与某些抗生素的报道相同。

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