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Advances in shotgun proteomics and the analysis of membrane proteomes

机译:shot弹枪蛋白质组学和膜蛋白质组学研究进展

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摘要

The emergence of shotgun proteomics has facilitated the numerous biological discoveries made by proteomic studies. However, comprehensive proteomic analysis remains challenging and shotgun proteomics is a continually changing field. This review details the recent developments in shotgun proteomics and describes emerging technologies that will influence shotgun proteomics going forward. In addition, proteomic studies of integral membrane proteins remain challenging due to the hydrophobic nature in integral membrane proteins and their general low abundance levels. However, there have been many strategies developed for enriching, isolating and separating membrane proteins for proteomic analysis that have moved this field forward. In summary, while shotgun proteomics is a widely used and mature technology, the continued pace of improvements in mass spectrometry and proteomic technology and methods indicate that future studies will have an even greater impact on biological discovery.
机译:shot弹枪蛋白质组学的出现促进了蛋白质组学研究的众多生物学发现。然而,全面的蛋白质组学分析仍然具有挑战性,shot弹枪蛋白质组学是一个不断变化的领域。这篇综述详细介绍了shot弹枪蛋白质组学的最新发展,并描述了将影响shot弹枪蛋白质组学的新兴技术。另外,由于整体膜蛋白的疏水性及其普遍的低丰度水平,整体膜蛋白的蛋白质组学研究仍然具有挑战性。然而,已经开发了许多用于富集,分离和分离用于蛋白质组学分析的膜蛋白的策略,这已经使该领域向前发展。总而言之,尽管shot弹枪蛋白质组学是一种广泛使用且成熟的技术,但质谱和蛋白质组学技术与方法的不断改进表明未来的研究将对生物学发现产生更大的影响。

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