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首页> 外文期刊>Journal of proteomics >Proteomlc analysis of the Actinidia deliciosa leaf apoplast during blotrophlc colonization by Pseudomonas syringae pv. actinidiae
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Proteomlc analysis of the Actinidia deliciosa leaf apoplast during blotrophlc colonization by Pseudomonas syringae pv. actinidiae

机译：丁香假单胞菌PV在菌落定殖过程中的美味猕猴桃叶质外体的蛋白质组学分析。猕猴桃

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摘要

For plant pathogenic bacteria, adaptation to the apoplast is considered as key in the establishment of the parasitic lifestyle. Pseudomonas syringae pv. acrinidiae (Psa), the causal agent of the bacterial canker of kiwifruit, uses leaves as the entry site to colonize plants. Through a combined approach based on 2-DE, nanoLC-ESI-LIT-MS/MS and quantitative PCR, we investigated Psa colonization of the Actinidia deliciosa "Hayward" leaf apoplast during the bacterial biotrophic phase. A total of 58 differentially represented protein species were identified in artificially inoculated leaves. Although the pathogen increased its population density during the initial period of apoplast colonization, plant defense mechanisms were able to impede further disease development. We identified a concerted action of different proteins mainly belonging to the plant defense and metabolism category, which intervened at different times and participated in reducing the pathogen population. On the other hand, bacterial BamA was highly represented during the first week of leaf apoplast colonization, whereas OmpA and Cpn60 were induced later. In addition to presenting further proteomic information on the molecular factors actively participating in this pathosystem, our data characterize the early events of host colonization and will promote the eventual development of novel bioassays for pathogen detection in kiwiplants.
机译：对于植物病原细菌，适应质外体被认为是建立寄生生活方式的关键。丁香假单胞菌PV。猕猴桃细菌性溃疡病的病因是草（Psa），它以叶子为定植点。通过基于2-DE，nanoLC-ESI-LIT-MS / MS和定量PCR的组合方法，我们研究了细菌生物营养阶段的猕猴桃“海沃德”叶质外体的Psa定殖。在人工接种的叶片中总共鉴定出58种差异表示的蛋白质种类。尽管病原体在质外体定殖的初期增加了种群密度，但植物防御机制却能够阻止疾病的进一步发展。我们确定了主要属于植物防御和代谢类别的不同蛋白质的协同作用，它们在不同时间进行干预并参与减少病原体种群。另一方面，细菌BamA在叶质外体定殖的第一周中表现出很高的代表性，而OmpA和Cpn60则随后被诱导。除了提供有关积极参与该病理系统的分子因素的蛋白质组学信息外，我们的数据还表征了宿主定殖的早期事件，并将促进猕猴桃中病原体检测的新型生物测定法的最终发展。

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