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首页> 外文期刊>Journal of liquid chromatography and related technologies >Capiliary electrophoresis of cytomegalovirus dna in an untreated capiliary
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Capiliary electrophoresis of cytomegalovirus dna in an untreated capiliary

机译:未经处理的毛细管中巨细胞病毒dna的毛细管电泳

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摘要

This work demonstrates that the PCR procucts of the Cytomegalovirus(CMV)can be deteced by capillary electrophoresis in uncoated capillaries using a zwitterionic buffer. Furthermore, dilution of the sample in phosphate buffer leads to stacking which improves the sensitivity by the ultraviolet detection without the need for sample desalting or cleanup.
机译:这项工作表明巨细胞病毒(CMV)的PCR产品可以使用两性离子缓冲液通过毛细管电泳在未包被的毛细管中进行检测。此外,样品在磷酸盐缓冲液中的稀释会导致堆积,从而通过紫外检测提高了灵敏度,而无需对样品进行脱盐或净化。

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