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首页> 外文期刊>Journal of Raman Spectroscopy: An International Journal for Original Work in All Aspects of Raman Spectroscopy, Including Higher Order Processes, and Also Brillouin- and Rayleigh Scattering >Using surface-enhanced Raman scattering (SERS) and fluorescence spectroscopy for screening yeast extracts, a complex component of cell culture media
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Using surface-enhanced Raman scattering (SERS) and fluorescence spectroscopy for screening yeast extracts, a complex component of cell culture media

机译：使用表面增强拉曼散射（SERS）和荧光光谱法筛选酵母提取物（细胞培养基的复杂成分）

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Yeastolate or yeast extract, which are hydrolysates produced by autolysis of yeast, are often employed as a raw material in the media used for industrial mammalian cell culture. The source and quality of yeastolate can significantly affect cell growth and production; however, analysis of these complex biologically derived materials is not straightforward. The best current method, liquid chromatography-mass spectrometry (LC-MS), is time-consuming and requires extensive expertise. This study describes the use of surface-enhanced Raman scattering (SERS) and fluorescence excitation-emission matrix (EEM) spectroscopy coupled with robust principal component analysis (ROBPCA) for the rapid and facile characterization and discrimination of yeast extracts in aqueous solution. SERS using silver colloids generates time-dependent signals, where adenine is the strongest contributor, and the spectra are stable and reproducible (< ～3%) at 180 min after mixing. Combining this spectral behavior with chemometric methods enables SERS to be used in discriminating between different yeastolate sources, for assessing lot-to-lot variability, and, potentially, to monitor storage-induced compositional changes. Fluorescence EEM combined with multiway ROBPCA also provides a rapid and inexpensive method for the discrimination of yeastolate, yielding results in terms of sample discrimination very similar to that obtained with SERS. However, the EEM data does not provide the same level of chemical information that is provided by the SERS. Thus, the combination of these two methodologies has the potential to be extremely useful in biopharmaceutical manufacturing, as well as for the rapid characterization and screening of biogenic hydrolysates from animal or plant sources.
机译：酵母酸盐或酵母提取物是通过酵母自溶而产生的水解产物，通常用作工业哺乳动物细胞培养所用培养基的原料。酵母酸盐的来源和质量会显着影响细胞的生长和产生;然而，对这些复杂的生物衍生材料的分析并不简单。目前最好的方法是液相色谱-质谱法（LC-MS），这很耗时，并且需要广泛的专业知识。这项研究描述了使用表面增强拉曼散射（SERS）和荧光激发发射矩阵（EEM）光谱结合稳健的主成分分析（ROBPCA）来快速，轻松地表征和区分水溶液中的酵母提取物。使用银胶体的SERS产生随时间变化的信号，其中腺嘌呤的贡献最大，并且混合后180分钟时光谱稳定且可重现（<〜3％）。将这种光谱行为与化学计量学方法相结合，可以将SERS用于区分不同的酵母酸盐源，评估批次间的变异性，并有可能监测储存引起的成分变化。荧光EEM与多路ROBPCA的结合还提供了一种快速，廉价的酵母菌鉴别方法，在样品鉴别方面产生的结果与SERS获得的结果非常相似。但是，EEM数据不能提供与SERS提供的相同水平的化学信息。因此，这两种方法的结合在生物制药生产以及快速表征和筛选动植物来源的生物水解产物方面具有极大的潜力。

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