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首页> 外文期刊>Clinical immunology: The official journal of the Clinical Immunology Society >Detection of IgE anti-parvovirus B19 and increased CD23+ B cells in parvovirus B19 infection: relation to Th(2) cytokines.
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Detection of IgE anti-parvovirus B19 and increased CD23+ B cells in parvovirus B19 infection: relation to Th(2) cytokines.

机译：检测细小病毒B19感染中的IgE抗细小病毒B19和增加的CD23 + B细胞：与Th（2）细胞因子有关。

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The immune profile of a parvovirus B19-infected patient (male, 8 years old) was studied on day 0 (initial presentation) and on days 14 and 210 post symptom presentation (psp). Before infection, the patient was skin test positive to various allergens, including ragweed and tree and grass pollens, and had a serum IgE level of 150 IU/mL. On day 0, the patient was diagnosed as parvovirus B19 infected, as judged by the presence of IgG anti-parvovirus Abs in serum (EIA) and presentation of "slap cheek" rash. The patient's serum IgE level increased from 150 IU/mL before infection to 256 IU/mL on day 0, was 233 IU/mL on day 14, and returned to preinfection levels on day 210. In contrast, there was little change in the levels of serum IgM, IgG, or IgA (nephelometry). IgE anti-parvovirus B19 protein (VP-N) was detected in serum (Western blot) on days 0, 14, and 210, despite the decrease in total IgE on day 210. Although there was no increase in total numbers of blood CD23+ B cells on day 0, by day 14 the numbersof these cells increased dramatically (93%), remaining high on day 210. In contrast, there were virtually no changes in total numbers of CD4+ and CD8+ T cells or CD16/56+ NK precursor cells on days 0-210. On day 0, when IgG and IgE anti-parvovirus were detected in serum, patient's peripheral blood mononuclear cells (PBMC) expressed mRNA for the Th2 cytokines IL-4 and IL-10, but not for the Th1 cytokines IFN-gamma or IL-2. However, by day 14 psp, PBMC expressed mRNA for the Th1 cytokines IFN-gamma and IL-2, as well as for IL-4 and IL-10. This is the first demonstration of the existence of IgE anti-parvovirus B19 Ab. The presence of IgE anti-parvovirus B19 Ab in serum on day 0 and its persistence in serum 7 months psp suggests that IgE anti-parvovirus may be useful in prognosis of parvovirus B19 infection. Our results reinforce the idea that IgE, in general, may play a major role in anti-viral immunity, perhaps in conjunction with CD23+ cells. The results further suggest that clearance of this infection is accompanied by a switch to Th1 cytokines.
机译：在第0天（初次出现）以及在症状出现后的第14和210天（psp）研究了细小病毒B19感染患者（8岁的男性）的免疫特征。感染前，患者的皮肤对各种过敏原（包括豚草，树木和草花粉）的皮肤测试呈阳性，血清IgE水平为150 IU / mL。在第0天，根据血清中存在IgG抗细小病毒Abs（EIA）并出现“巴掌”皮疹的情况判断，该患者被诊断为细小病毒B19感染。患者的血清IgE水平从感染前的150 IU / mL增加到第0天的256 IU / mL，在第14天为233 IU / mL，并在第210天恢复到感染前水平。相比之下，该水平几乎没有变化血清IgM，IgG或IgA（比浊法）。尽管在第210天总IgE有所下降，但在第0、14和210天仍在血清中（免疫印迹）检测到IgE抗细小病毒B19蛋白（VP-N）。尽管血液中CD23 + B的总数没有增加第0天的细胞，到第14天，这些细胞的数量急剧增加（93％），在第210天保持高水平。相比之下，CD4 +和CD8 + T细胞或CD16 / 56 + NK前体细胞的总数几乎没有变化在0-210天。在第0天，当在血清中检测到IgG和IgE抗细小病毒时，患者的外周血单核细胞（PBMC）表达Th2细胞因子IL-4和IL-10的mRNA，而不表达Th1细胞因子IFN-γ或IL- 2。然而，到第14天时，PBMC表达Th1细胞因子IFN-γ和IL-2以及IL-4和IL-10的mRNA。这是IgE抗细小病毒B19 Ab存在的第一个证明。 IgE抗细小病毒B19 Ab在第0天的血清中存在并且在血清中持续存在7个月psp提示IgE抗细小病毒可能在细小病毒B19感染的预后中有用。我们的研究结果强化了这样一个想法，即IgE通常可以与CD23 +细胞一起在抗病毒免疫中起主要作用。结果进一步表明清除这种感染伴随着向Th1细胞因子的转换。

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