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首页> 外文期刊>Clinical immunology: The official journal of the Clinical Immunology Society >Antigen-specific cytometry-New tools arrived!
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Antigen-specific cytometry-New tools arrived!

机译：抗原特异性细胞仪-新工具到来了！

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摘要

Until recently, immunofluorescence-based cytometry and cell sorting, which have now found their place in the repertoire of state-of-the-art technologies, have mostly served to identify and assess subsets of leukocytes and thereby to evaluate rather systemic changes of the immune system. A more detailed defined evaluation of immune responses was not possible for a long time. In particular, a focus of the cytometric analysis on those lymphocytes specifically recognizing a defined antigen was hampered due to technical limitations. Yet, traditional methods for the analysis of antigen-specific lymphocytes typically relied upon measurements of proliferation or cytokine expression in bulk cultures. In recent times, this hindrance has been overcome both for B and T lymphocytes. We review here the emerging field of antigen-specific cytometry and describe the most widely used state of the art and future technologies that offer exciting new options to analyze and isolate specifically those lymphocytes that are directly involved in the immune reaction to given antigens, options that have already spurred research, diagnosis, and therapy beyond scope.
机译：直到最近，基于免疫荧光的细胞计数和细胞分选已在最先进的技术中找到了自己的位置，主要用于鉴定和评估白细胞的亚群，从而评估免疫系统的相当大的变化。系统。长期以来不可能对免疫应答进行更详细的定义评估。特别地，由于技术限制，阻碍了对那些特异性识别确定的抗原的淋巴细胞的细胞计数分析的重点。然而，用于分析抗原特异性淋巴细胞的传统方法通常依赖于在大量培养物中增殖或细胞因子表达的测量。最近，对于B和T淋巴细胞都克服了这一障碍。我们在这里回顾了抗原特异性细胞计数法的新兴领域，并描述了使用最广泛的技术和未来技术，这些技术提供了令人兴奋的新选择，可以专门分析和分离那些直接参与针对给定抗原的免疫反应的淋巴细胞。已经刺激了超出范围的研究，诊断和治疗。

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