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首页> 外文期刊>Clinical immunology: The official journal of the Clinical Immunology Society >The secretory IFN-gamma response of single CD4 memory cells after activation on different antigen presenting cell types.
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The secretory IFN-gamma response of single CD4 memory cells after activation on different antigen presenting cell types.

机译：在不同抗原呈递细胞类型上激活后，单个CD4记忆细胞的分泌性IFN-γ反应。

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It is unknown to what extent the heterogeneity of antigen presenting cells (APC) influences the IFN-gamma response of CD4 memory cells. We re-stimulated DO11.10 T cell receptor (TCR)-transgenic cells and wild-type CD4 memory cells with OVA-peptide 323-339 presented on purified dendritic cells (DC), macrophages, and B cells. Using IFN-gamma ELISPOT assays, we measured the number of cytokine producing T cells and the amount of cytokine produced by individual T cells at different time points after antigen encounter. The data showed that, when CD4 cells recognized antigen on DC, the induction of cytokine production was accelerated compared to macrophages and B cells. In contrast, the per-cell cytokine productivity was independent of the type of APC by which the T cells were re-stimulated. Moreover, the peptide concentration required for CD4 cell activation was comparable for the different APC. The data suggest that DC induce cytokine production in memory cells with accelerated activation kinetics, whereas 24 h of antigen stimulation on DC, macrophages, and B cells results in comparable levels of T cell activation. These data have implications for the understanding of T cell memory responses when T cells re-encounter antigen on different APC as well as for the monitoring of memory T cell responses ex vivo.
机译：抗原呈递细胞（APC）的异质性在多大程度上影响CD4记忆细胞的IFN-γ反应尚不清楚。我们用纯化树突状细胞（DC），巨噬细胞和B细胞上呈现的OVA肽323-339重新刺激了DO11.10 T细胞受体（TCR）转基因细胞和野生型CD4记忆细胞。使用IFN-γELISPOT分析，我们测量了抗原接触后不同时间点产生细胞因子的T细胞的数量以及单个T细胞产生的细胞因子的数量。数据显示，当CD4细胞识别DC上的抗原时，与巨噬细胞和B细胞相比，细胞因子产生的诱导被加速了。相反，每细胞的细胞因子生产率与重新刺激T细胞的APC类型无关。而且，CD4细胞活化所需的肽浓度对于不同的APC是相当的。数据表明，DC以加速的激活动力学诱导记忆细胞中细胞因子的产生，而DC，巨噬细胞和B细胞上24小时的抗原刺激导致T细胞激活的水平相当。这些数据对理解T细胞在不同APC上遇到抗原时的T细胞记忆反应以及对离体记忆T细胞反应的监测具有重要意义。

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