Products of anti-CD3/anti-CD28 activated lymphocytes induce differentiation and maturation of dendritic cells and have adjuvant-like activity in vitro and in vivo.

Harris KM; Lenz P; Hankey KG; MacVittie T; Farese A; Nakajima K; Hasumi K; Mann DL

首页> 外文期刊>Clinical immunology: The official journal of the Clinical Immunology Society >Products of anti-CD3/anti-CD28 activated lymphocytes induce differentiation and maturation of dendritic cells and have adjuvant-like activity in vitro and in vivo.

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Products of anti-CD3/anti-CD28 activated lymphocytes induce differentiation and maturation of dendritic cells and have adjuvant-like activity in vitro and in vivo.

机译：抗CD3 /抗CD28活化的淋巴细胞的产物诱导树突状细胞的分化和成熟，并在体外和体内具有佐剂样活性。

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Dendritic cells (DC) orchestrate immune responses under direction of cytokines/chemokines in their microenvironment. To investigate the influence of that generated during T cell activation, we stimulated peripheral blood mononuclear cells (PBMC) with anti-CD3 and anti-CD28 coated beads and tested cell-free culture supernatants (lymphocyte conditioned medium, LCM) for cytokine/chemokine composition and biologic activity. LCM contained a battery of mediators important in the biology of myeloid (mDC) and plasmacytoid (pDC) DC. LCM differentiated monocytes into functional immature mDC, and induced maturation of immature mDC. LCM also augmented maturation and IFNalpha-production of CpG-treated pDC. Functional activity of LCM-derived DC was confirmed by their ability to enhance in vitro recall T cell responses and substantially augment in vivo cellular and humoral immune responses to various vaccines in non-human primates. These results demonstrate that products of anti-CD3/anti-CD28 stimulated PBMC generatebiologically active DC in vitro and function as a vaccine adjuvant in vivo.

机译：树突状细胞（DC）在其微环境中的细胞因子/趋化因子的指导下协调免疫反应。为了研究T细胞活化过程中产生的影响，我们用抗CD3和抗CD28包被的珠刺激了外周血单核细胞（PBMC），并测试了无细胞培养上清液（淋巴细胞条件培养基，LCM）的细胞因子/趋化因子组成和生物活性。 LCM包含一系列在髓样（mDC）和浆细胞样（pDC）DC生物学中重要的介体。 LCM将单核细胞分化为功能性未成熟的mDC，并诱导未成熟的mDC成熟。 LCM还增强了CpG处理的pDC的成熟度和IFNα的产生。 LCM衍生的DC增强体外召回T细胞反应并显着增强体内对非人灵长类动物中各种疫苗的细胞和体液免疫反应的能力证实了其功能活性。这些结果证明，抗CD3 /抗CD28刺激的PBMC的产物在体外产生生物活性DC，并且在体内用作疫苗佐剂。

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《Clinical immunology: The official journal of the Clinical Immunology Society》 |2008年第1期|共11页
作者
Harris KM; Lenz P; Hankey KG; MacVittie T; Farese A; Nakajima K; Hasumi K; Mann DL;
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正文语种 eng
中图分类医学免疫学;
关键词
Adjuvants; Immunologic; Animals; Antibodies; Antigens; CD28; Antigens; CD3; 佐剂; 免疫; 动物; 抗体; 抗原; CD28; 抗原; CD3; 细胞分化; 培养基; 条件性;

机译：Adjuvants;Immunologic;Animals;Antibodies;Antigens;CD28;Antigens;CD3;佐剂;免疫;动物;抗体;抗原;CD28;抗原;CD3;细胞分化;培养基;条件性;

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1. Products of anti-CD3/anti-CD28 activated lymphocytes induce differentiation and maturation of dendritic cells and have adjuvant-like activity in vitro and in vivo. [J] . Harris KM, Lenz P, Hankey KG, Clinical immunology: The official journal of the Clinical Immunology Society . 2008,第1期

机译：抗CD3 /抗CD28活化的淋巴细胞的产物诱导树突状细胞的分化和成熟，并在体外和体内具有佐剂样活性。
2. Antitumor reactivity of anti-CD3/anti-CD28 bead-activated lymphoid cells: implications for cell therapy in a murine model. [J] . Ito F, Carr A, Svensson H, Journal of immunotherapy . 2003,第3期

机译：抗CD3 /抗CD28珠激活的淋巴样细胞的抗肿瘤反应性：对小鼠模型中细胞治疗的影响。
3. Vaccination with dendritic cells pulsed in vitro with tumor antigen conjugated to cholera toxin efficiently induces specific tumoricidal CD8(+) cytotoxic lymphocytes dependent on cyclic AMP activation of dendritic cells. [J] . Sun JB, Eriksson K, Li BL, Clinical immunology: The official journal of the Clinical Immunology Society . 2004,第1期

机译：用与霍乱毒素偶联的肿瘤抗原体外脉冲刺激的树突状细胞的疫苗有效诱导特定的CD8（+）细胞杀伤性肿瘤细胞，依赖于树突状细胞的环AMP活化。
4. Regulation of Ganoderma lucidum Polysaccharides on Cytotoxic T Lymphocytes Induced by Dendritic Cells in vitro [C] . H.He, rnP.Stroeven, rnZ.Guo, Ganoderma: Genetics, Chemistry, Pharmacology and Therapeutics . 2002

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5. The role of paired box 5, B lymphocyte-induced maturation protein-1 and activation protein-1 in the suppression of B cell differentiation by 2,3,7,8-tetrachlorodibenzo-p-dioxin [D] . Schneider, Dina 2008

机译：配对盒5，B淋巴细胞诱导的成熟蛋白-1和激活蛋白-1在2,3,7,8-四氯二苯并-p-二恶英抑制B细胞分化中的作用
6. Bovine WC1+ and WC1neg γδ T Lymphocytes Influence Monocyte Differentiation and Monocyte-Derived Dendritic Cell Maturation during In Vitro Mycobacterium avium Subspecies paratuberculosis Infection [O] . Monica M. Baquero, Brandon L. Plattner -1

机译：牛WC1 +和WC1negγδT淋巴细胞在鸟分枝杆菌亚种副结核病感染过程中影响单核细胞分化和单核细胞衍生的树突状细胞成熟。
7. Depending on their maturation state, splenic dendritic cells induce the differentiation of CD4(+) T lymphocytes into memory and/or effector cells in vivo. [O] . De Heusch, Magali, Oldenhove, Guillaume, Urbain, Jacques, 2004

机译：取决于它们的成熟状态，脾树突状细胞在体内诱导CD4（+）T淋巴细胞分化为记忆和/或效应细胞。
8. T-Lymphocyte Maturation: Cell Surface Markers and Immune Function Induced by T-Lymphocyte Cell-Free Products and Thymosin Polypeptides [R] . Ahmed, A., Wong, D. M., Thurman, G. B., 1979

机译：T淋巴细胞成熟：T淋巴细胞无细胞产物和胸腺素多肽诱导的细胞表面标志物和免疫功能

Products of anti-CD3/anti-CD28 activated lymphocytes induce differentiation and maturation of dendritic cells and have adjuvant-like activity in vitro and in vivo.

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