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首页> 外文期刊>Journal of Plant Nutrition >Effects of nitrogen and sulfur on total phenolics and antioxidant activity in two genotypes of leaf mustard.
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Effects of nitrogen and sulfur on total phenolics and antioxidant activity in two genotypes of leaf mustard.

机译：氮和硫对两种基因型芥菜总酚和抗氧化活性的影响。

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Leaf mustard (Brassica juncea Coss) is widely used for both fresh and processed markets in southern China. It contains high nutritional and medicinal compounds, which are important for maintaining optimum health. The objective of this study was to determine the influence of nitrogen (N) and sulfur (S) nutrition on total phenolics and antioxidant activity in two genotypes of leaf mustard (cvs. 'Xuelihong' and 'Zhujie'). Plants were greenhouse-grown using nutrient solutions with two levels of nitrogen (10 and 25 mM) and three levels of sulfur (0.5, 1, and 2 mM). Total phenolic concentrations were considerably decreased by increasing nitrogen supply, whereas increased by increasing sulfur supply. Total phenolic concentrations in cv 'Zhujie' was higher than in cv 'Xuelihong'. Three assays including 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity, beta -carotene bleaching (BCB), and ferric reducing antioxidant power (FRAP) were used to evaluate antioxidant activity. Increasing nitrogen supply reduced DPPH radical scavenging activity and FRAP value, but increased antioxidant activity using BCB assay. Increasing sulfur supply increased antioxidant activity with all three tests. The effects of genotype on DPPH radical scavenging activity and FRAP value were not significant, however, antioxidant activity using BCB assay was significantly higher in cv 'Zhujie' than in cv 'Xuelihong'. A significantly positive correlation was found between DPPH radical scavenging activity and total phenolic concentrations in two genotypes, FRAP value and total phenolic concentrations in cv 'Xuelihong'.
机译：芥菜（Brassica juncea Coss）在中国南方的新鲜市场和加工市场均被广泛使用。它含有高营养和药用化合物，对于保持最佳健康状况非常重要。这项研究的目的是确定氮（N）和硫（S）营养对两种基因型芥菜（品种“雪力红”和“朱洁”）总酚和抗氧化活性的影响。使用具有两个氮含量（10和25 mM）和三个硫含量（0.5、1和2 mM）的营养液使植物温室生长。总氮浓度通过增加氮供应而大大降低，而总硫浓度通过增加硫供应而增加。品种'竹节'中的总酚浓度高于品种'雪里红'。三种测定方法包括1,1-二苯基-2-吡啶并肼基（DPPH）清除自由基活性，β-胡萝卜素漂白（BCB）和还原铁抗氧化能力（FRAP）用于评估抗氧化活性。氮供应的增加降低了DPPH自由基清除活性和FRAP值，但使用BCB分析增加了抗氧化活性。在所有三个测试中，增加硫的供应都会增加抗氧化活性。基因型对DPPH自由基清除活性和FRAP值的影响不显着，但是，使用“ BCJ”法测定的“朱街”品种的抗氧化活性明显高于“薛里红”品种。发现两种基因型的DPPH自由基清除活性与总酚浓度，cv“学力红”中的FRAP值和总酚浓度之间存在显着正相关。

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