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首页> 外文期刊>Clinical immunology: The official journal of the Clinical Immunology Society >Cloning, expression and characterization of heat shock protein 60 (groEL) of Salmonella enterica serovar Typhi and its role in protective immunity against lethal Salmonella infection in mice.
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Cloning, expression and characterization of heat shock protein 60 (groEL) of Salmonella enterica serovar Typhi and its role in protective immunity against lethal Salmonella infection in mice.

机译：鼠伤寒沙门氏菌热休克蛋白60（groEL）的克隆，表达和表征及其在小鼠抗致命沙门氏菌感染的保护性免疫中的作用。

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摘要

Heat shock proteins (Hsps) represent dominant antigens in numerous microbial infections, suggesting a potential use of pathogen-derived Hsps for vaccination. The present study evaluates the immunogenicity and protective efficacy of groEL (Hsp60) of Salmonella enterica serovar Typhi against lethal challenge by S. Typhi Ty2 and Salmonella enterica serovar Typhimurium in mice. The groEL gene was cloned and expressed in Escherichia coli BL21 and purified by affinity chromatography. Immunization of mice with groEL resulted in a significant increase in antibody titers. Antibody isotyping revealed that groEL immunization induces both IgG1 and IgG2a antibodies. There was a significant increase in lymphocyte proliferation, interleukin-4 and interferon-gamma levels in cells isolated from immunized mice as compared to control. Immunization of mice with recombinant groEL protein with or without adjuvant conferred 70-90% protection against lethal infections either by S. Typhi Ty2 or S. Typhimurium. Passive immunization with anti-groEL sera also protected 50% mice against lethal infection.
机译：热激蛋白（Hsps）代表了许多微生物感染中的优势抗原，表明潜在地使用了病原体衍生的Hsps进行疫苗接种。本研究评估了鼠伤寒沙门氏菌的groEL（Hsp60）对鼠伤寒沙门氏菌Ty2和鼠伤寒沙门氏菌的致死性攻击的免疫原性和保护作用。将groEL基因克隆并在大肠杆菌BL21中表达，并通过亲和层析纯化。用groEL免疫小鼠会导致抗体效价的显着提高。抗体分型显示groEL免疫诱导IgG1和IgG2a抗体。与对照相比，从免疫小鼠分离的细胞中淋巴细胞增殖，白介素-4和干扰素-γ水平显着增加。用重组groEL蛋白（有或没有佐剂）对小鼠进行免疫，可对伤寒沙门氏菌Ty2或伤寒沙门氏菌造成70-90％的致死性保护。抗groEL血清的被动免疫还可以保护50％的小鼠免于致命感染。

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