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首页> 外文期刊>Clinical immunology: The official journal of the Clinical Immunology Society >A human anti-HIV autoantibody enhances EBV transformation and HIV infection.
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A human anti-HIV autoantibody enhances EBV transformation and HIV infection.

机译：人类抗HIV自身抗体可增强EBV转化和HIV感染。

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A highly specific, human IgG mAb, F223, which reacts with both HIV-1-infected cells and uninfected lymphoid cells, has been derived. F223 reacts with gp120 but fails to neutralize viral infection. The antibody does enhance HIV-1 infection in a complement-dependent manner. The autoantigen recognized by F223 is expressed on a small percentage of T cells and NK cells and the majority of B cells. Immunoprecipitation demonstrates F223 reactivity with an as of yet unidentified 159-kDa protein in uninfected lymphoid cells. This reactivity with uninfected cells is inhibited by free gp120 demonstrating the cross-reactive nature of this antibody. The F223 light chain demonstrates strong homology to VLlambda2 family genes whereas the heavy chain is most homologous (84%) to the germline gene VH3-H.11. In vivo usage of VH3 family genes by F223 and an anti-HIV-1 (gp41) human mAb, 3D6, with related autoreactivity, suggests that VH3 sequences may be important components of potentially pathogenic human anti-HIV-1 envelope autoantibodies. F223 was isolated from an HIV-1 infected individual with lymphoma and in vitro F223 significantly enhances EBV transformation of normal B cells and increases immunoglobulin production without affecting B cell proliferation. Characterization of this antibody response may provide important insights and mechanistic information on HIV pathogenesis. Copyright 1999 Academic Press.
机译：已经获得了一种高度特异性的人IgG mAb F223，它与HIV-1感染的细胞和未感染的淋巴样细胞均发生反应。 F223与gp120反应，但不能中和病毒感染。该抗体确实以补体依赖性方式增强HIV-1感染。 F223识别的自身抗原在小比例的T细胞和NK细胞以及大多数B细胞上表达。免疫沉淀表明F223与未感染的淋巴样细胞中迄今尚未鉴定的159-kDa蛋白反应。游离gp120抑制了与未感染细胞的这种反应性，这证明了该抗体的交叉反应性。 F223轻链表现出与VLlambda2家族基因的强同源性，而重链与种系基因VH3-H.11最同源（84％）。 F223和具有相关自身反应性的抗HIV-1（gp41）人类mAb，3D6在体内使用VH3家族基因表明，VH3序列可能是潜在致病性人类抗HIV-1包膜自身抗体的重要组成部分。 F223是从感染了HIV-1的淋巴瘤患者中分离出来的，在体外F223可显着增强正常B细胞​​的EBV转化并增加免疫球蛋白的产生，而不会影响B细胞的增殖。此抗体反应的特征可能提供有关HIV发病机理的重要见解和机制信息。版权所有1999，学术出版社。

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