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首页> 外文期刊>Clinical immunology: The official journal of the Clinical Immunology Society >Normalized CD8+ but not CD4+ lymphocyte IL-2 expression is associated with early treatment with highly active antiretroviral therapy.
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Normalized CD8+ but not CD4+ lymphocyte IL-2 expression is associated with early treatment with highly active antiretroviral therapy.

机译：标准化的CD8 +而不是CD4 +淋巴细胞IL-2表达与高活性抗逆转录病毒疗法的早期治疗有关。

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CD4+ and CD8+ lymphocyte cytokine production in patients with HIV/AIDS and Controls, in response to stimulation with phorbol-12-myristate-13-acetate (PMA) and ionomycin was assessed using single cell flow cytometric methods. Sixty-eight patients with HIV were divided into those on no antiretroviral therapy and those on highly active antiretroviral therapy (HAART). Patients on HAART were analyzed further on the basis of gender, ethnicity, viral load (> or 100 or <100 cells/mm(3)) and CD4 count (>200 or <200 cells/mm(3)). Interferon gamma (IFNgamma) expression by CD4+ and CD8+ lymphocytes was elevated in HIV-infected groups as compared to Controls. This elevation was statistically significant for patients on HAART but not for those not on HAART. The most significant difference was seen when the CD4+ count reached >200 cells/mm(3) (p=0.018 for CD4+ IFNgamma production and p=0.004 for CD8+ IFNgamma production). CD4+ interleukin-2 (IL-2) expression was significantly lower in HIV patients as compared to Controls but did not significantly improve however good the response to HAART. IL-2 expression by CD8+ lymphocytes was also lower in HIV patients as compared to Controls. IL-2 expression by CD8+ lymphocytes significantly improved in all patients on HAART as compared to HIV patients on no HAART. IL-2 expression was not significantly different from that of the Controls when the HIV viral load was less than 50 copies/ml. These results demonstrate improvements in both CD4+ and CD8+ responsiveness with HAART. IFNgamma production was elevated in response to HAART and was maximal only with significant CD4 count recovery. In contrast, normalization of IL-2 production by CD8+ lymphocytes was seen early in patients receiving HAART even when there was only a small increase in CD4+ lymphocyte numbers.
机译：使用单细胞流式细胞术评估了接受phorbol-12-肉豆蔻酸酯13-乙酸酯（PMA）和离子霉素刺激的HIV / AIDS和对照组患者CD4 +和CD8 +淋巴细胞的细胞因子。 68例HIV患者分为未接受抗逆转录病毒治疗的患者和接受高活性抗逆转录病毒治疗（HAART）的患者。根据性别，种族，病毒载量（>或 100或<100个细胞/ mm（3））和CD4计数（ > 200或<200个/ mm（3））。与对照组相比，HIV感染组中CD4 +和CD8 +淋巴细胞的干扰素γ（IFNγ）表达升高。对于使用HAART的患者，此升高具有统计学意义，但对于未使用HAART的患者则无统计学意义。当CD4 +计数达到> 200细胞/ mm（3）时，可以看到最显着的差异（对于CD4 +IFNγ产生，p = 0.018;对于CD8 + IFNgamma产生，p = 0.004）。与对照组相比，HIV患者中CD4 +白细胞介素2（IL-2）的表达明显降低，但对HAART的反应却没有明显改善。与对照组相比，HIV患者中CD8 +淋巴细胞的IL-2表达也较低。与没有HAART的HIV患者相比，所有HAART的患者CD8 +淋巴细胞的IL-2表达均得到明显改善。当HIV病毒载量小于50拷贝/ ml时，IL-2表达与对照组无显着差异。这些结果证明，HAART可以改善CD4 +和CD8 +的响应能力。响应HAART，IFNgamma产量升高，并且只有在CD4计数显着恢复时才达到最大值。相反，在接受HAART的患者中，早期可观察到CD8 +淋巴细胞使IL-2产生正常化，即使CD4 +淋巴细胞的数量仅小幅增加。

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