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首页> 外文期刊>Clinical immunology: The official journal of the Clinical Immunology Society >The clinical course of EAE is reflected by the dynamics of the neuroantigen-specific T cell compartment in the blood.
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The clinical course of EAE is reflected by the dynamics of the neuroantigen-specific T cell compartment in the blood.

机译：血液中神经抗原特异性T细胞区室的动态反映了EAE的临床过程。

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摘要

Due to the limited numbers of PBMCs that can be obtained from the blood of individual mice, the key question whether central disease parameters such as onset, progression and severity correlate with the magnitude and cytokine quality of the T cell response in experimental autoimmune encephalomyelitis (EAE) has remained unanswered. Here we introduce an ELISPOT-based PBMC test system in which as little as 150 mul of murine blood are sufficient, allowing to bleed mice repeatedly while continuing to observe the clinical course of EAE. Using this technique, we demonstrate that longitudinal measurements of antigen-specific IFN-gamma and IL-17 production in the blood are a highly suitable approach to predict the disease outcome in remitting-relapsing PLP:139-151- and chronic MOG:35-55-induced EAE of SJL/J and C57BL/6 mice, respectively. Our data propound cytokine monitoring as promising tool in the quest for more efficient diagnostic and prognostic options in human multiple sclerosis and other autoimmune diseases.
机译：由于可以从个别小鼠的血液中获得的PBMC数量有限，因此关键问题是中心性疾病参数（例如发作，进展和严重程度）是否与实验性自身免疫性脑脊髓炎（EAE）中T细胞反应的大小和细胞因子质量相关）仍未得到答复。在这里，我们介绍一种基于ELISPOT的PBMC测试系统，其中仅150 mul的鼠血就足够了，可以在继续观察EAE的临床过程的情况下反复使小鼠流血。使用这项技术，我们证明了对血液中抗原特异性IFN-γ和IL-17产生的纵向测量是预测在复发型PLP：139-151-和慢性MOG：35-中疾病结局的高度合适的方法55诱导的SJL / J和C57BL / 6小鼠的EAE。我们的数据提示细胞因子监测是有希望的工具，可用于寻求更有效的人类多发性硬化症和其他自身免疫性疾病的诊断和预后选择。

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