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首页> 外文期刊>Journal of Reproductive Immunology >Fas ligand expression by maternal decidual cells is negatively correlated with the abundance of leukocytes present at the maternal-fetal interface.
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Fas ligand expression by maternal decidual cells is negatively correlated with the abundance of leukocytes present at the maternal-fetal interface.

机译：母体蜕膜细胞的Fas配体表达与母体-胎儿界面处存在的白细胞丰富度负相关。

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摘要

The abundance of leukocytes at the maternal-fetal interface could influence the fate and invasion of extravillous trophoblasts. However, the mechanism(s) involved in determining the number of leukocytes present at the maternal-fetal interface as well as the nature of the interactions between invading fetal trophoblasts, maternal leukocytes and decidual cells are not well understood. In the present studies, we examined Fas ligand (FasL)/Fas expression at the maternal-fetal interface in human placental tissues of early pregnancy by immunohistochemistry. The types of cells and their localization were also characterized by specific cell markers (cytokeratin, vimentin and CD45 for trophoblast, decidual cells and leukocytes, respectively). The cells undergoing apoptosis and specific apoptotic trophoblasts were detected by TUNEL assay and M30 cytoDEATH immunostaining, respectively. Using single or double immunostaining, we found that FasL expression in decidual cells was negatively correlated with the number of Fas-expressing leukocytes in the same region. Furthermore, the density of leukocytes had an inverse relationship with the number of interstitial trophoblasts present in the same area. We observed also that extravillous trophoblasts are viable despite expressing Fas and being in close proximity to decidual cell-derived FasL. These data support our hypothesis that maternal decidual cell-derived FasL may be involved in preventing the recruitment of Fas-bearing leukocytes at the maternal-fetal interface through apoptosis induction by Fas/FasL interaction, thereby promoting trophoblast invasion.
机译：母胎界面上的白细胞丰富会影响绒毛外滋养层细胞的命运和侵袭。然而，对于确定存在于母胎界面上的白细胞数量以及侵入的胎儿滋养层细胞，母体白细胞和蜕膜细胞之间相互作用的性质所涉及的机制尚不清楚。在本研究中，我们通过免疫组织化学检查了早孕人胎盘组织中母胎界面的Fas配体（FasL）/ Fas表达。细胞的类型及其定位还通过特定的细胞标记物（滋养细胞，蜕膜细胞和白细胞的细胞角蛋白，波形蛋白和CD45）来表征。分别通过TUNEL法和M30 cytoDEATH免疫染色法检测凋亡细胞和特定的凋亡滋养细胞。使用单次或两次免疫染色，我们发现蜕膜细胞中FasL的表达与同一区域表达Fas的白细胞的数量呈负相关。此外，白细胞的密度与同一区域中存在的间质滋养细胞的数量成反比。我们还观察到，尽管表达Fas且与蜕膜细胞衍生的FasL十分接近，但绒毛外滋养层细胞仍是可行的。这些数据支持了我们的假说，即母体蜕膜细胞衍生的FasL可能通过Fas / FasL相互作用诱导细胞凋亡，从而阻止母胎界面上Fas携带的白细胞募集，从而促进滋养层细胞的侵袭。

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