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首页> 外文期刊>Journal of Reproductive Immunology >Human endogenous retrovirus K (HERV-K) is expressed in villous and extravillous cytotrophoblast cells of the human placenta.
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Human endogenous retrovirus K (HERV-K) is expressed in villous and extravillous cytotrophoblast cells of the human placenta.

机译：人内源性逆转录病毒K（HERV-K）在人胎盘的绒毛和绒毛外滋养层细胞中表达。

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摘要

Human endogenous retroviruses (HERVs) have been shown to be important in physiological and pathophysiological processes in humans. Several HERVs have been found to be expressed in the placenta-a tissue with special immunomodulatory functions that is responsible for nutrition of the embryo and the ability of the semiallogenic trophoblast to invade. The envelope proteins of HERV-W (also known as syncytin 1) and HERV-FRD (syncytin 2) were shown to be involved in cell fusion leading to the generation of the syncytiotrophoblast. Syncytin 2 was further shown to have immunosuppressive properties. Herein we analyse the expression of another HERV, HERV-K, which is characterised by open reading frames for all viral genes. Using immunohistochemistry and Western blot analysis, expression of the transmembrane envelope (TM) protein of HERV-K was studied in normal placental and decidual tissues obtained at different gestational ages. The TM protein was expressed exclusively in villous (VT) and extravillous cytotrophoblast (EVT) cells, but not in the syncytiotrophoblast or other cells. The expression of the TM protein of HERV-K in EVT cells was confirmed by Western blot analysis of isolated c-erbB2-expressing cytotrophoblast cells. Thus, this is the first report showing expression of the TM protein of HERV-K in normal human placental tissue with an exclusive expression in cytotrophoblast cells, suggesting a potential involvement of HERV-K in placentogenesis and pregnancy. Since retroviral TM proteins including the TM protein of HERV-K have immunosuppressive properties, expression of the TM protein of HERV-K may contribute to immune protection of the fetus.
机译：人类内源性逆转录病毒（HERV）已显示在人类的生理和病理生理过程中很重要。已发现在具有特殊免疫调节功能的胎盘组织中表达了几种HERV，这与胚胎的营养和半同种异体滋养细胞侵袭的能力有关。 HERV-W（也称为合体素1）和HERV-FRD（合体素2）的包膜蛋白显示参与细胞融合，导致合体滋养层细胞的生成。 Syncytin 2进一步显示具有免疫抑制特性。本文中，我们分析了另一种HERV HERV-K的表达，其特征是所有病毒基因的开放阅读框。使用免疫组织化学和蛋白质印迹分析，研究了在不同胎龄获得的正常胎盘和蜕膜组织中HERV-K的跨膜包膜（TM）蛋白的表达。 TM蛋白仅在绒毛（VT）和绒毛外滋养层细胞（EVT）细胞中表达，而在合体滋养层细胞或其他细胞中不表达。通过对分离的表达c-erbB2的成细胞细胞进行Western印迹分析，证实了EVT细胞中HERV-K TM蛋白的表达。因此，这是首次报道HERV-K TM蛋白在正常人胎盘组织中的表达而在滋养层细胞中的排他性表达，这表明HERV-K可能参与胎盘发生和妊娠。由于包括HERV-K的TM蛋白的逆转录病毒TM蛋白具有免疫抑制特性，因此HERV-K的TM蛋白的表达可能有助于胎儿的免疫保护。

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