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首页> 外文期刊>Journal of Reproductive Immunology >Analysis of B cell epitopes of a glycoprotein porcine zona pellucida (pZP1).
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Analysis of B cell epitopes of a glycoprotein porcine zona pellucida (pZP1).

机译：糖蛋白猪透明带（pZP1）的B细胞表位分析。

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摘要

The zona pellucida (ZP) of mammalian oocytes forms an extracellular matrix composed of three major glycoproteins and plays an important role in sperm-zona interactions. As ZP had a strong organ-specific but species-cross-reactive antigenicity and passive or active immunization with ZP antigens could impair fertilization, the possibility of developing a immunocontraceptive vaccine has been extensively studied. Studies on active immunization with porcine ZP (pZP) that contain B cell epitopes and T cell epitopes demonstrated that a temporary infertility could be induced along with the elevation of antibody titers, but it was always associated with ovarian failure. This could be due to the oophoritis by activation of pathogenic T cell immunity. It is the general consideration that any adverse effects by vaccination should be avoided for an immunocontraception. From this point of view, the analysis of B cell epitopes of pZP protein would be helpful for construction of a safe immunocontraceptive vaccine with zona antigens. We determined the amino acid sequence of the B epitope in the pZP1 protein by using a monoclonal antibody (MAb-5H4) that possesses a fertilization blocking ability. In addition, antiserum raised to the epitope sequence was revealed to block in vitro fertilization of homologous animal species.
机译：哺乳动物卵母细胞的透明带（ZP）形成由三种主要糖蛋白组成的细胞外基质，并在精子-精子相互作用中起重要作用。由于ZP具有很强的器官特异性但物种交叉反应的抗原性，并且用ZP抗原进行被动或主动免疫可能会损害受精，因此已广泛研究了开发免疫避孕疫苗的可能性。包含B细胞表位和T细胞表位的猪ZP（pZP）主动免疫的研究表明，随着抗体效价的升高，可以诱导暂时性不育，但它总是与卵巢衰竭有关。这可能是由于致病性T细胞免疫激活引起的卵巢炎。一般认为，免疫避孕应避免接种疫苗引起的任何不良反应。从这个角度出发，分析pZP蛋白的B细胞表位将有助于构建具有透明带抗原的安全的免疫避孕疫苗。我们通过使用具有受精阻断功能的单克隆抗体（MAb-5H4），确定了pZP1蛋白中B表位的氨基酸序列。另外，揭示出升高至表位序列的抗血清阻断了同源动物物种的体外受精。

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