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首页> 外文期刊>Journal of Reproductive Immunology >Characterization of the subsets of human NKT-like cells and the expression of Th1/Th2 cytokines in patients with unexplained recurrent spontaneous abortion
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Characterization of the subsets of human NKT-like cells and the expression of Th1/Th2 cytokines in patients with unexplained recurrent spontaneous abortion

机译：不明原因反复自然流产患者NKT样细胞亚群的特征及Th1 / Th2细胞因子的表达

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The objective was to investigate the subsets of natural killer T (NKT)-like cells and the expression of Th1/Th2 cytokines in the peripheral blood (PB) and/or decidual tissue of patients with unexplained recurrent spontaneous abortion (URSA). The percentages of NKT-like cells in the PB and deciduas of URSA patients in early pregnancy and in the PB of nonpregnant women were analyzed by flow cytometry. The expression of interferon (IFN)-gamma (Th1 cytokine) and Th2 cytokines, interleukin (IL)-4 and IL-10, in the PB and decidual tissue was measured by quantitative RT-PCR and enzyme-linked immunosorbent assay (ELISA). Most percentages of subsets of NKT-like cells (CD3(+)CD56(+), CD3(+)CD56(+)CD16(+)) in the PB and deciduas were significantly greater in URSA patients than in normal pregnant and nonpregnant women. A cut-off value of 3.75% for the increased percentage of CD3(+)CD56(+)CD16(+) NKT-like cells in the PB appeared to be predictive of pregnancy failure. Moreover, we found that in the decidua, IFN-gamma expression was significantly higher, while IL-4 and IL-10 expression was significantly lower in URSA patients compared with those with a normal pregnancy. The ratio of decidual Th1/Th2 cytokines in URSA patients was significantly increased compared with that in normal pregnant women. Decidual IL-4 expression correlated negatively with the percentages of blood CD3(+)CD56(+)CD16(+) NKT-like cells and the decidual CD3(+)CD56(+) and CD3(+)CD56(+)CD16(+) NKT-like cells. NKT-like cells may play an important role in maintaining normal pregnancy. Measurement of CD3(+)CD56(+)CD16(+) NKT-like cells in the PB may provide a potential tool for assessing patients' risk of spontaneous abortion. (C) 2015 Published by Elsevier Ireland Ltd.
机译：目的是研究无法解释的反复自然流产（URSA）患者外周血（PB）和/或蜕膜组织中自然杀伤T（NKT）样细胞的亚群和Th1 / Th2细胞因子的表达。通过流式细胞术分析了URSA患者在怀孕早期的PB和蜕皮中的NKT样细胞的百分比以及未怀孕妇女的PB中的NKT样细胞的百分比。通过定量RT-PCR和酶联免疫吸附测定（ELISA）测定PB和蜕膜组织中干扰素（IFN）-γ（Th1细胞因子）和Th2细胞因子，白介素（IL）-4和IL-10的表达。 。与正常孕妇和非孕妇相比，URSA患者中PB和蜕皮病的NKT样细胞亚群（CD3（+）CD56（+），CD3（+）CD56（+）CD16（+））的百分比最高。 PB中CD3（+）CD56（+）CD16（+）NKT样细胞百分比增加的临界值为3.75％，似乎可预测妊娠失败。此外，我们发现与正常妊娠相比，URSA患者的蜕膜中的IFN-γ表达显着较高，而IL-4和IL-10的表达显着较低。与正常孕妇相比，URSA患者的蜕膜Th1 / Th2细胞因子比例显着增加。蜕膜IL-4表达与血液CD3（+）CD56（+）CD16（+）NKT样细胞以及蜕膜CD3（+）CD56（+）和CD3（+）CD56（+）CD16（ +）NKT样细胞。 NKT样细胞可能在维持正常妊娠中起重要作用。 PB中CD3（+）CD56（+）CD16（+）NKT样细胞的测量可能为评估患者自发流产的风险提供了一种潜在的工具。 （C）2015由爱思唯尔爱尔兰有限公司出版。

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